Genetic incorporation of L-dihydroxyphenylalanine (DOPA) biosynthesized by a tyrosine phenol-lyase
Genetic incorporation of L-dihydroxyphenylalanine (DOPA) biosynthesized by a tyrosine phenol-lyase
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DOI：
10.1039/c8cc00281a
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发表时间：
2018-03-25
影响因子：
4.9
通讯作者：
Lee, Hyun Soo
中科院分区：
文献类型：
--
作者：
Kim, Sanggil;Sung, Bong Hyun;Lee, Hyun Soo
利用酪氨酸-苯酚裂解酶在大肠杆菌中以邻苯二酚、丙酮酸和氨为原料合成L-二羟基苯丙氨酸（DOPA），并将合成的氨基酸直接整合到蛋白质中。用含有DOPA的突变蛋白进行的三个生化实验证实了生物合成的DOPA的遗传掺入，并揭示了其在各种生化应用中的潜力。
L-Dihydroxyphenylalanine (DOPA) was biosynthesized by a tyrosine-phenol lyase from catechol, pyruvate, and ammonia in Escherichia coli, and the biosynthesized amino acid was directly incorporated into proteins. Three biochemical experiments with mutant proteins containing DOPA confirmed the genetic incorporation of biosynthesized DOPA, and revealed its potential for various biochemical applications.