Comparison of secretory protein and membrane composition of secretory granules isolated from normal and neoplastic pancreatic acinar cells of rats.
Comparison of secretory protein and membrane composition of secretory granules isolated from normal and neoplastic pancreatic acinar cells of rats.
复制标题
从大鼠正常和肿瘤性胰腺腺泡细胞中分离的分泌颗粒的分泌蛋白和膜组成的比较。
DOI：
10.1073/pnas.80.14.4379
复制
发表时间：
1983
影响因子：
11.1
通讯作者：
Reddy,JK
中科院分区：
文献类型：
--
作者：
Hansen,LJ;Reddy,MK;Reddy,JK
在可移植的大鼠胰腺腺泡癌细胞中，细胞分化的多样性为研究不同于正常腺泡细胞的调控分子事件提供了生物模型系统。比较肿瘤细胞和正常胰腺腺泡细胞的分泌(酶原)颗粒蛋白和颗粒膜，以确定明显分化的含有分泌颗粒的肿瘤细胞和正常细胞基因表达的差异。在正常分泌颗粒提取液的二维聚丙烯酰胺凝胶中观察到的19种蛋白质也出现在肿瘤颗粒提取液的轮廓中，脂肪酶和4种碱性蛋白的量减少但可检测到。此外，肿瘤颗粒提取物含有一种新的mr 24000蛋白质，命名为p24，这在正常提取物中是检测不到的。尽管肿瘤颗粒膜具有与正常膜相似的磷脂成分，但缺少或含有数量大大减少的主要糖蛋白MR 80,000和其他三种蛋白质MRS 50,000，37,000和36,000。分泌颗粒提取物中p24蛋白的性质以及MR 80,000膜糖蛋白在该肿瘤中的减少或缺失的意义仍有待阐明。
The diversity of cytodifferentiation in a transplantable rat pancreatic acinar carcinoma provides a biological model system for the study of regulatory molecular events that differ from those in normal acinar cells. Secretory (zymogen) granule proteins and granule membranes of neoplastic and normal pancreatic acinar cells were compared to determine the differences in gene expression between apparently well-differentiated secretory granule-containing neoplastic cells and normal cells. Nineteen proteins observed in two-dimensional polyacrylamide gels of normal secretory granule extracts were seen also in tumor granule extract profiles, with reduced but detectable amounts of lipase and four basic proteins. In addition, tumor granule extracts contained a new protein of Mr 24,000, designated p24, which was not detectable in normal extracts. Neoplastic granule membranes, while having phospholipid composition similar to that of normal membranes, lacked or contained a greatly reduced amount of a major glycoprotein of Mr 80,000 and three other proteins of Mrs 50,000, 37,000, and 36,000. The nature of p24 protein in secretory granule extracts and the significance of the reduction or absence of Mr 80,000 membrane glycoprotein in this tumor remain to be elucidated.