Dysfunction of microRNA-32 regulates ubiquitin ligase FBXW7 in multiple myeloma disease.
Dysfunction of microRNA-32 regulates ubiquitin ligase FBXW7 in multiple myeloma disease.
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DOI：
10.2147/ott.s105945
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发表时间：
2016
影响因子：
4
通讯作者：
Yang L
中科院分区：
文献类型：
--
作者：
Hua J;Ding T;Yang L
microRNA （miRNA）表达功能障碍与肿瘤的发生、进展和发展有关。这项工作的目的是研究miR-32 -一种在不同肿瘤中异常调节的miRNA -在多发性骨髓瘤（MM）患者的临床组织中的功能障碍。我们评估miR-32表达水平的肿瘤组织是在我们5年的临床实践中收集的。我们的研究发现miR-32在MM组织中的表达增加。对MM组织中F-box和WD重复结构域7 （FBXW7）的评估显示，FBXW7的表达与miR-32呈负相关。为了进一步研究miR-32与FBXW7之间的关系，我们用miR-32或anti-miR-32转染细胞。体外研究发现，转染miR-32的细胞FBXW7表达较低，癌症相关蛋白c-Jun和c-Myc表达较高。相比之下，转染anti-miR32的细胞FBXW7的表达相对较高，而c-Jun和c-Myc的表达较低。本研究可能为MM癌的检测和治疗提供新的策略。
Dysfunction of microRNA (miRNA) expression has been associated with tumor occurrence, progression, and development. The aim of this work was to study the dysfunction of miR-32 – an miRNA that was abnormally regulated in different tumors – in clinical tissues from patients with multiple myeloma (MM). The tumor tissues in which we assessed miR-32 expression levels were collected during our 5 years of clinical practice. Our study found an increase in miR-32 expression in MM tissues. Assessment of F-box and WD repeat domain-containing 7 (FBXW7) in MM tissues showed an inverse relation between the expression of FBXW7 and miR-32. To further investigate the relation between miR-32 and FBXW7, cells were transfected with miR-32 or anti-miR-32. In vitro studies found that cells transfected with miR-32 showed a lower expression of FBXW7 and a higher expression of cancer-related proteins, c-Jun and c-Myc. In contrast, the cells transfected with anti-miR32 showed a relatively higher expression of FBXW7, but a lower expression of c-Jun and c-Myc. This study may offer perceptive insights into developing new strategies for MM cancer detection and therapy.