Structure, inter-annual recurrence, and global-scale connectivity of airborne microbial communities
Structure, inter-annual recurrence, and global-scale connectivity of airborne microbial communities
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DOI：
10.1016/j.scitotenv.2014.04.030
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发表时间：
2014-07-15
影响因子：
9.8
通讯作者：
Casamayor, Emilio O.
中科院分区：
文献类型：
--
作者：
Barberan, Albert;Henley, Jessica;Casamayor, Emilio O.
来自地球上大沙漠的灰尘，如撒哈拉沙漠，可以传播很长的距离，散布在数千平方公里的范围内。由于全球变化，远距离灰尘沉积率正在增加，可能是洲际微生物扩散的一种机制。偏远的贫营养高山湖泊对灰尘输入特别敏感，可以作为空中微生物运输和加速洲际微生物迁移的生态后果的哨兵。在这项研究中，我们采用高通量测序技术（16 S rRNA扩增子焦磷酸测序），以表征沿着三年收集在比利牛斯山脉中部（西班牙东北部）的大气沉积物的微生物群落。此外，还对毛里塔尼亚土壤和高海拔比利牛斯湖空气-水界面的细菌进行了研究。在气溶胶沉积的社区随时间变化的年际相似性强的季节性成分。来自同一季节的群落比来自不同季节的群落更相似。反过来，来自不同日期的样品稍微倾向于具有更多不同的微生物组合（即，时间距离衰减），总的来说，这表明大气沉积可能会影响汇生境在时间上可预测的方式。三个栖息地检查（土壤，沉积物，空气-水界面）窝藏不同的微生物群落，虽然空气中收集的样品在比利牛斯山脉在撒哈拉沙漠沙尘暴期间更接近撒哈拉沙漠的土壤样品比那些收集在没有撒哈拉沙漠沙尘发作。这三个栖息地共享的c.a.占数据集中微生物序列总数的1.4%，这些成功的移民在不同的细菌类别中传播。总的来说，这项研究表明，当地和区域的特点可能会产生全球趋势的动态和分布的空气微生物组合，并在高层大气中的活细胞的多样性可能高于先前的预期。(C)2014爱思唯尔有限公司版权所有。
Dust coming from the large deserts on Earth, such as the Sahara, can travel long distances and be dispersed over thousands of square kilometers. Remote dust deposition rates are increasing as a consequence of global change and may represent a mechanism for intercontinental microbial dispersal. Remote oligotrophic alpine lakes are particularly sensitive to dust inputs and can serve as sentinels of airborne microbial transport and the ecological consequences of accelerated intercontinental microbial migration. In this study, we applied high-throughput sequencing techniques (16S rRNA amplicon pyrosequencing) to characterize the microbial communities of atmospheric deposition collected in the Central Pyrenees (NE Spain) along three years. Additionally, bacteria from soils in Mauritania and from the air-water interface of high altitude Pyrenean lakes were also examined. Communities in aerosol deposition varied in time with a strong seasonal component of interannual similarity. Communities from the same season tended to resemble more each other than those from different seasons. Samples from disparate dates, in turn, slightly tended to have more dissimilar microbial assemblages (i.e., temporal distance decay), overall suggesting that atmospheric deposition may influence sink habitats in a temporally predictable manner. The three habitats examined (soil, deposition, and air-water interface) harbored distinct microbial communities, although airborne samples collected in the Pyrenees during Saharan dust outbreaks were closer to Mauritian soil samples than those collected during no Saharan dust episodes. The three habitats shared c.a. 1.4% of the total number of microbial sequences in the dataset Such successful immigrants were spread in different bacterial classes. Overall, this study suggests that local and regional features may generate global trends in the dynamics and distribution of airborne microbial assemblages, and that the diversity of viable cells in the high atmosphere is likely higher than previously expected. (C) 2014 Elsevier B.V. All rights reserved.