Genetic and phylogenetic analysis of the non-structural proteins NS1, NS2 and NS3 of epizootic haemorrhagic disease virus (EHDV)
Genetic and phylogenetic analysis of the non-structural proteins NS1, NS2 and NS3 of epizootic haemorrhagic disease virus (EHDV)
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DOI：
10.1016/j.virusres.2009.07.019
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发表时间：
2009-11-01
期刊：
影响因子：
5
通讯作者：
Mertens, P. P. C.
中科院分区：
文献类型：
--
作者：
Anthony, S. J.;Maan, N.;Mertens, P. P. C.
流行性出血症病毒(EHDV)在感染宿主细胞过程中产生三种独特的非结构蛋白：NS1、NS2和NS3。这项研究提供了对这些蛋白质(以及它们的编码基因)的完整遗传和系统发育分析，以比较作用于每种蛋白质的选择压力。登录号、基因和蛋白质大小、ORF位置、G+C含量、末端六核苷酸、起始和终止密码子以及系统发育关系。与核心蛋白或外壳蛋白不同，所有EHDV NS蛋白没有共同的特征遗传或系统发育特征；这表明每种蛋白都在不同的选择压力下进化。对这些差异进行了讨论。还提供了基因组片段8(编码NS2)基因重组的证据，以及基因复制和突变的证据，表明EHDV基因组可能是使用这样的机制进化的。(C)2009爱思唯尔B.V.保留所有权利。
Three unique non-structural (NS) proteins are produced by Epizootic haemorrhagic disease virus (EHDV) during infection of a host cell; NS1, NS2 and NS3. This study presents a complete genetic and phylogenetic analysis of these proteins (and the genes that code for them) to allow comparison of the selective pressures acting on each. Accession numbers, gene and protein sizes, ORF positions, G + C contents, terminal hexanucleotides, start and stop codons and phylogenetic relationships are all presented. Unlike the core, or outer-coat proteins, there are no characteristic genetic or phylogenetic traits common to all of the EHDV NS proteins; indicating that each is evolving under different selection pressures. These differences are discussed. Evidence of genetic recombination in genome segment 8 (coding for NS2) is also presented, together with evidence of gene duplication and mutation, suggesting the EHDV genome may have evolved using mechanisms such as these. (C) 2009 Elsevier B.V. All rights reserved.