Optimization of a comprehensive two-dimensional normal-phase and reversed-phase liquid chromatography system
Optimization of a comprehensive two-dimensional normal-phase and reversed-phase liquid chromatography system
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DOI：
10.1093/chromsci/44.9.561
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发表时间：
2006-10-01
影响因子：
1.3
通讯作者：
Mondello, Luigi
中科院分区：
文献类型：
--
作者：
Dugo, Paola;Fernandez, Maria del Mar Ramirez;Mondello, Luigi
本研究基于在方法优化过程中对综合二维液相色谱 (LCxLC) 系统性能的评估。 LCxLC 装置在第一维 (11 D) 中以正相 (NP) 模式（吸附）运行，在第二维 (2D) 中以反相 (RP) 模式运行，配备 1D 微径硅胶色谱柱和 2D 整体式 C-18 色谱柱，并以 10 通双位阀作为接口。二维分离后使用光电二极管阵列检测器。解决了由于二维中使用的流动相的不混溶性而导致峰失真的可能原因。使用各种标准化合物对两个维度和二维初始梯度的分析运行时间和流速进行优化。这种 LCxLC 方法的潜力和多功能性通过 11 种标准成分（其中大部分是过敏原）的分离得到了证明。后者的特点是在二维空间平面上分散分布，在疏水性和极性的基础上进行分离。
The present investigation is based on the evaluation of the performance of a comprehensive two-dimensional liquid chromatography (LCxLC) system during method optimization. The LCxLC set-up, operated in normal phase (NP) mode (adsorption) in the first dimension (11 D) and reversed-phase (RP) mode in the second dimension (2D), is equipped with a 1D microbore silica column and a 2D monolithic C-18 column with a 10-port two position valve as the interface. A photodiode array detector is used after the 2D separation. A possible cause of peak distorsion because of the immiscibility of the mobile phases employed in the two dimensions is resolved. The optimization of the analytical run time and flow rate for both dimensions and the initial gradient in the 2D is carried out with various standard compounds. The potential and versatility of this LCxLC approach is demonstrated through the separation of 11 standard components, most of them allergens. The latter, which are characterized by a scattered distribution on the 2D space plane, underwent separation on both a hydrophobicity and polarity basis.