Tissue and fixative dependent shifts of δ13C and δ15N in preserved ecological material
Tissue and fixative dependent shifts of δ13C and δ15N in preserved ecological material
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DOI：
10.1002/rcm.1661
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发表时间：
2004-01-01
影响因子：
2
通讯作者：
Jennings, S
中科院分区：
文献类型：
--
作者：
Sweeting, CJ;Polunin, NVC;Jennings, S
碳和氮稳定同位素分析通常用于调查水生食物网，并在使用存档材料的回顾性调查中具有潜在的应用。然而，这种分析假设储存不会改变保存材料的同位素特征，或者储存期间同位素组成的变化是可预测的。在这里，我们研究保存在80%的乙醇，4%的甲醛，冷冻后21个月的鳕鱼（Gadus morhua）肌肉，鱼卵和肝脏组织的保存变化。保存的变化是不一致的组织。在4%甲醛中，高蛋白组织比低蛋白组织表现出更大的δ（15）N位移，而在酒精中保存时，相对较高的脂肪组织发生更大的δ（13）C位移。冷冻并没有改变同位素特征。δ（15）N和δ（13）C的反应可以通过防腐剂的同位素特征和反应特性以及保存组织的生化组成的差异来解释。结果澄清了一些过程中，导致同位素变化保存。版权所有（C）2004约翰威利父子有限公司。
Carbon and nitrogen stable isotope analyses are routinely used to investigate aquatic food webs, and have potential application in retrospective investigations using archived materials. However, such analyses assume that storage does not alter isotopic signatures of materials preserved, or that changes in isotopic composition during storage are predictable. Here we examine preservation shifts on cod (Gadus morhua) muscle, roe and liver tissue over 21 months following preservation in 80% ethanol, in 4% formaldehyde, and by freezing. Preservation shifts were not consistent among tissues. High protein tissues exhibited greater delta(15)N shifts than low protein tissues in 4% formaldehyde, while greater delta(13)C shifts occurred in relatively higher fat tissues when preserved in alcohol. Freezing did not change isotopic signatures. Responses of delta(15)N and delta(13)C are explained by differences in the preservative's isotopic signature and the reaction properties and biochemical composition of the tissues preserved. The results clarify some of the processes that lead to isotopic change during preservation. Copyright (C) 2004 John Wiley Sons, Ltd.