Toward Using Fluorescent Nanodiamonds To Study Chronological Aging in Saccharomyces cerevisiae.
Toward Using Fluorescent Nanodiamonds To Study Chronological Aging in Saccharomyces cerevisiae.
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DOI：
10.1021/acs.analchem.8b03431
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发表时间：
2018-10
影响因子：
7.4
通讯作者：
K. van der Laan;J. Naulleau;V. Damle;A. Sigaeva;Nicolas Jamot;Felipe P. Perona-Martinez;M. Chipaux;R. Schirhagl
中科院分区：
文献类型：
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作者：
K. van der Laan;J. Naulleau;V. Damle;A. Sigaeva;Nicolas Jamot;Felipe P. Perona-Martinez;M. Chipaux;R. Schirhagl
旨在解释细胞衰老的理论之一是衰老的自由基理论，该理论描述了自由基的产生和积累增加的可能作用。荧光纳米金刚石（FND）被提出来提供一种检测这些自由基的工具，因为它们用作磁性传感器，根据它们的磁性环境改变它们的光学特性。因此，它们可以在分子水平上研究衰老，并揭示自由基在这一过程中的确切作用。在这项研究中，朝着这一目标迈出了重要的一步。将FND引入按时间老化的酵母细胞中。此外，研究了FND在这些老化细胞中的行为，以证明使用FND寻找细胞老化原因的潜力。
One of the theories aiming to explain cellular aging is the free radical theory of aging, which describes the possible role of increased production and accumulation of free radicals. Fluorescent nanodiamonds (FNDs) are proposed to provide a tool to detect these radicals, as they function as magnetic sensors that change their optical properties depending on their magnetic surrounding. Therefore, they could enable the study of aging at a molecular level and unravel the exact role of free radicals in this process. In this study, important steps toward this goal are made. FNDs are introduced in chronologically aging yeast cells. Furthermore, the behavior of FNDs in these aging cells is studied to demonstrate the potency of using FNDs in the search for causes of cellular aging.