Variability in the Viral Protein Linked to the Genome of Turnip Mosaic Virus Influences Interactions with eIF(iso)4Es in Brassica rapa.
Variability in the Viral Protein Linked to the Genome of Turnip Mosaic Virus Influences Interactions with eIF(iso)4Es in Brassica rapa.
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与萝卜花叶病毒基因组相关的病毒蛋白的变异影响与白菜中 eIF(iso)4E 的相互作用
DOI：
10.5423/ppj.oa.07.2020.0125
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发表时间：
2021-02-01
期刊：
影响因子：
--
通讯作者：
Sun R
中科院分区：
文献类型：
--
作者：
Li G;Zhang S;Li F;Zhang H;Zhang S;Zhao J;Sun R
植物通过被动和主动抗性机制保护免受病毒的侵害，并且在迄今为止表征的大多数情况下，对马铃薯Y病毒属的天然隐性抗性已被定位于真核起始因子eIF 4 E或eIF（iso）4 E基因中的突变。在芜菁中检测到5个eIF 4 E拷贝和3个eIF（iso）4 E拷贝。eIF 4 E和eIF（iso）4 E基因可以与连接到基因组（VPg）的芜菁花叶病毒（TuMV）病毒蛋白相互作用，启动病毒翻译。酵母双杂交（Y2 H）和双分子荧光互补（BiFC）分析表明，TuMV-CHN 2/CHN 3 VPgs不能与BraA. eIF（iso）4 E. a/c或BraA.eIF（iso）4 E. c相互作用，但能与B中的BraA.eIF（iso）4 E. a相互作用。拉帕。进一步分析表明，TuMV-UK 1 VPg中L186 F（nt T556 C）的氨基酸替换对构建TuMV-UK 1 VPg与eIF（iso）4 E蛋白的相互作用网络具有重要意义。BraA的相互作用模型。构建了含有TuMV VPg的eIF（iso）4 E蛋白，并通过分析其氨基酸序列，推测了TuMV VPgs-eIF（iso）4 E相互作用的影响，特别是TuMV-UK 1 VPg中的L186 F是否改变了TuMV-UK 1 VPg蛋白的结构，从而终止了TuMV VPg蛋白与BraA.eIF（iso）4 E的相互作用。该研究为植物病毒与翻译起始因子的相互作用提供了新的视角，揭示了关键氨基酸的作用机制。
Plants protect against viruses through passive and active resistance mechanisms, and in most cases characterized thus far, natural recessive resistance to potyviruses has been mapped to mutations in the eukaryotic initiation factor eIF4E or eIF(iso)4E genes. Five eIF4E copies and three eIF(iso)4E copies were detected in Brassica rapa. The eIF4E and eIF(iso)4E genes could interact with turnip mosaic virus (TuMV) viral protein linked to the genome (VPg) to initiate virus translation. From the yeast two-hybrid system (Y2H) and bimolecular fluorescence complementation (BiFC) assays, the TuMV-CHN2/CHN3 VPgs could not interact with BraA.eIF4E.a/c or BraA.eIF(iso)4E.c, but they could interact with BraA.eIF(iso)4E.a in B. rapa. Further analysis indicated that the amino acid substitution L186F (nt T556C) in TuMV-UK1 VPg was important for the interaction networks between the TuMV VPg and eIF(iso)4E proteins. An interaction model of the BraA. eIF(iso)4E protein with TuMV VPg was constructed to infer the effect of the significant amino acids on the interaction of TuMV VPgs-eIF(iso)4Es, particularly whether the L186F in TuMV-UK1 VPg could change the structure of the TuMV-UK1 VPg protein, which may terminate the interaction of the BraA.eIF(iso)4E and TuMV VPg protein. This study provides new insights into the interactions between plant viruses and translation initiation factors to reveal the working of key amino acids.