Modeling HIV transfer between dendritic cells and T cells: importance of HIV phenotype, dendritic cell-T cell contact and T-cell activation.
Modeling HIV transfer between dendritic cells and T cells: importance of HIV phenotype, dendritic cell-T cell contact and T-cell activation.
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模拟树突状细胞和 T 细胞之间的 HIV 转移：HIV 表型、树突状细胞-T 细胞接触和 T 细胞激活的重要性。
DOI：
10.1097/00002030-200010200-00011
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Rich,E
中科院分区：
文献类型：
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作者：
Vanham,G;Penne,L;Allemeersch,H;Kestens,L;Willems,B;vanderGroen,G;Jeang,KT;Toossi,Z;Rich,E
ObjectiveTo study the requirements for HIV transfer between dendritic cells (DC) and CD4 T cells, using an in vitro model, combined with flow cytometry.MethodsImmature DC and macrophages (MA) were generated from monocytes. After infection, DC or MA were cultured alone or with purified CD4 T cells. Intracellular HIV was measured, using (1) the monocyte (MO)-tropic AD8 HIV, endowed with enhanced green fluorescent protein (EGFP); and (2) intracellular staining of laboratory HIV strains and clones from primary isolates.Results(1) Clone AD8-EGFP infected DC and MA with equal efficiency, but the virus was preferentially transferred from DC to autologous T cells.(2) DC were more productively infected with R5/NSI, as compared to X4/SI, HIV, but both HIV phenotypes were easily transmitted to autologous T4 cells.(3) HIV-infected DC transferred the virus to T cells across a semi-permeable membrane, if the T cells were in contact with non-infected DC.(4) Co-culture of T cells with autologous non-infected DC induced T-cell activation. HIV-infected DC selectively increased HLA-DR on T cells and HLA-DR (+) T cells were preferential targets for HIV transfer.(5) Resting Ba-L-infected CD4 T cells were able to transmit the virus ‘inversely’to co-cultured DC.ConclusionHIV transfer between monocyte-derived dendritic cells and autologous CD4 T cells was directly demonstrated using flow cytometry. The transfer proceeded in both directions, depended on cellular contact and was associated with partial T-cell activation. This model, representing relevant in vivo targets of HIV, is useful to further investigate interactions between HIV, DC and T cells, without the need for primary ex vivo DC.
