Polyamine oxidase and acrolein as novel biochemical markers for diagnosis of cerebral stroke
Polyamine oxidase and acrolein as novel biochemical markers for diagnosis of cerebral stroke
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DOI：
10.1161/01.str.0000190004.36793.2d
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发表时间：
2005-12-01
期刊：
影响因子：
8.3
通讯作者：
Igarashi, K
中科院分区：
文献类型：
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作者：
Tomitori, H;Usui, T;Igarashi, K
背景和目的 - 我们以前发现，丙烯醛（CH2 = CHCHO）和精子氧化酶（SMO）的血浆水平与慢性肾功能衰竭的严重程度很好地相关。这项研究的目的是测试中风患者的血浆中这两种标记和乙酰丙二胺氧化酶（ACPAO）的水平是否增加。方法 - ACPAO和SMO的活性以及蛋白质与蛋白偶联的丙烯醛水平中风患者和正常受试者分别通过高性能液相色谱和ELISA进行测量。通过MRI或计算机断层扫描（CT）估算局灶性梗塞。折叠 - 中风患者血浆中ACPAO，SMO和丙烯醛的水平显着增加。中风的大小几乎与丙烯醛和总多胺氧化酶（ACPAO Plus SMO）的乘以值相似。中风发作后，首次增加了ACPAO，然后是SMO的水平和最后的丙烯醛水平升高。在1例情况下，ACPAO和SMO的增加在MRI或CT检测到的局灶性损伤之前。此外，在许多轻度症状的患者（11例）中，MRI证实了中风，他们的总多胺氧化酶和丙烯醛水平升高。在明显的正常受试者（8例）中，丙烯醛X总多胺氧化酶的值很高，MRI.Conclusions在4例中发现了中风 - 结果表明，ACPAO，SMO和Acrolein的水平升高是中风的良好标志。
Background and Purpose - We found previously that plasma levels of acrolein (CH2 = CHCHO) and spermine oxidase (SMO) were well correlated with the degree of severity of chronic renal failure. The aim of this study was to test whether the levels of these 2 markers and of acetylpolyamine oxidase (AcPAO) were increased in the plasma of stroke patients.Methods - The activity of AcPAO and SMO and the level of protein-conjugated acrolein in plasma of the stroke patients and normal subjects were measured by high-performance liquid chromatography and ELISA, respectively. Focal infarcts were estimated by MRI or computed tomography (CT).Results - The levels of AcPAO, SMO, and acrolein were significantly increased in the plasma of stroke patients. The size of stroke was nearly parallel with the multiplied value of acrolein and total polyamine oxidase (AcPAO plus SMO). After the onset of stroke, an increase in AcPAO first occurred, followed by increased levels of SMO and finally acrolein. In 1 case, an increase in AcPAO and SMO preceded focal damage as detected by MRI or CT. Furthermore, stroke was confirmed by MRI in a number of mildly symptomatic patients (11 cases) who had increased levels of total polyamine oxidase and acrolein. Among apparently normal subjects (8 cases) who had high values of acrolein x total polyamine oxidase, stroke was found in 4 cases by MRI.Conclusions - The results indicate that increased levels of AcPAO, SMO, and acrolein are good markers of stroke.