An insulin-dependent hypoglycaemia induced by electroacupuncture at the Zhongwan (CV12) acupoint in diabetic rats
An insulin-dependent hypoglycaemia induced by electroacupuncture at the Zhongwan (CV12) acupoint in diabetic rats
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DOI：
10.1007/s001250051146
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发表时间：
1999-02-01
期刊：
影响因子：
8.2
通讯作者：
Cheng, JT
中科院分区：
文献类型：
--
作者：
Chang, SL;Lin, JG;Cheng, JT
针灸中脘穴在中医学中被广泛用于缓解糖尿病症状。本研究观察了电针中脘穴对糖尿病大鼠血糖的影响。还使用放射免疫测定法测定胰岛素、胰高血糖素和β-内啡肽的血浆浓度。电针（15 Hz，10 mA）中脘穴30 min后，大鼠血糖明显降低。这在正常大鼠和II型（非胰岛素依赖型）糖尿病大鼠模型中观察到。在I型（胰岛素依赖型）糖尿病大鼠模型中未观察到对血糖的显著影响;无论是链脲佐菌素（STZ）诱导的糖尿病大鼠还是遗传（BB/W）大鼠。此外，电针刺激的低血糖作用消失在大鼠胰岛素抵抗诱导的人长效胰岛素注射每天重复，导致甲苯磺丁脲诱导的低血糖的损失。因此，可以假设胰岛素相关作用。这一假设得到了支持，在正常大鼠电针刺激后，血浆胰岛素样免疫反应性的增加。排除了胰高血糖素的参与，因为电针刺激没有引起血浆胰高血糖素样免疫反应性的变化。除血浆β-内啡肽样免疫反应性增加外，足够剂量的纳洛酮阻断阿片受体可取消电针刺激中脘穴的降糖作用。因此，我们认为，电针刺激中脘穴诱导分泌内源性β-内啡肽，从而降低血糖浓度的胰岛素依赖性方式。
Acupuncture at the Zhongwan acupoint has been widely used in traditional Chinese medicine to relieve symptoms of diabetes mellitus. Our study investigated the effect on plasma glucose of electroacupuncture applied at the Zhongwan acupoint in rat diabetic models. Plasma concentrations of insulin, glucagon and beta-endorphin- were also determined using radioimmunoassay. A decrease in plasma glucose was observed in rats after electroacupuncture (15 Hz, 10 mA) for 30 min at the Zhongwan acupoint. This was observed in normal rats and rat models with Type II (non-insulin-dependent) diabetes mellitus. No significant effect on plasma glucose was observed in rat models with Type I (insulin-dependent) diabetes mellitus; neither the streptozotocin (STZ)-induced diabetic rats nor the genetic (BB/W) rats. Further, the hypoglycaemic action of electroacupuncture stimulation disappeared in rats with insulin-resistance induced by an injection of human long-acting insulin repeated daily to cause the loss of tolbutamide-induced hypoglycaemia. An insulin-related action can thus be hypothesised. This hypothesis is supported by an increase in plasma insulin-like immunoreactivity after electroacupuncture stimulation in normal rats. Participation of glucagon was ruled out because there was no change in plasma glucagonlike immunoreactivity resulting from electroacupuncture stimulation. In addition to an increase in plasma beta-endorphin-like immunoreactivity, the plasma glucose lowering action of electroacupuncture stimulation at Zhongwan acupoint was abolished by naloxone in a sufficient dose to block opioid receptors. Thus we suggest that electroacupuncture stimulation at the Zhongwan acupoint induces secretion of endogenous beta-endorphin which reduces plasma glucose concentration in an insulin-dependent manner.