Prevention of Necrotizing Enterocolitis through Milk Polar Lipids Reducing Intestinal Epithelial Apoptosis.
Prevention of Necrotizing Enterocolitis through Milk Polar Lipids Reducing Intestinal Epithelial Apoptosis.
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DOI：
10.1021/acs.jafc.0c02629
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发表时间：
2020-06
影响因子：
6.1
通讯作者：
Yujie Yang;Tao Zhang;Guangyu Zhou;Xiaoxiao Jiang;Mingxuan Tao;Jiaxin Zhang;Xiaoqun Zeng;Zhen Wu;D. Pan;Yuxing Guo
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文献类型：
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作者：
Yujie Yang;Tao Zhang;Guangyu Zhou;Xiaoxiao Jiang;Mingxuan Tao;Jiaxin Zhang;Xiaoqun Zeng;Zhen Wu;D. Pan;Yuxing Guo
新生儿坏死性小肠结肠炎（NEC）是一种常见且严重的疾病。本研究的目的是探讨牛奶极性脂质（MPLs）对LPS诱导的肠道炎症和细胞凋亡的保护机制。采用超高效液相色谱-串联质谱法（UPLC-MS/MS）对酪乳中的MPLs进行分析。采用苏木精-伊红染色法（H&E）和TUNEL法（Terminal Deoxynucleotidyl Transferase-mediated dUTP-biotin Nick-End Labeling assay）观察肠黏膜形态学变化和肠上皮细胞凋亡情况，结果表明MPLs可减轻NEC症状和肠上皮细胞凋亡。MPLs组IL-6、IL-8、TNF-α表达明显下调（P<0.05），IL-10表达明显上调（P<0.05）。同时，MPLs还能显著降低LPS诱导的TLR 4/ NF-κB信号通路的激活（P <0.05）。此外，MPLs通过降低Bax、Caspase-9和Caspase-3的表达以及增加Bcl-2的表达来抑制细胞凋亡。总之，MPLs可以通过抑制细胞炎症和保护肠道细胞凋亡来减轻小鼠NEC症状。
Neonatal necrotizing enterocolitis (NEC) is a common and devastating disease. The objective of this research was to investigate the protective mechanisms of Milk polar lipids (MPLs) on the attenuation of LPS-induced intestinal inflammation and apoptosis. MPLs were extracted from buttermilk and analyzed using Ultra Performance Liquid Chromatography (UPLC)-MS/MS. Neonatal NEC rat model was used to investigate the effects of MPLs on NEC and its underlying mechanisms. H&E (Hematoxylin-eosin staining) and TUNEL (Terminal Deoxynucleotidyl Transferase-mediated dUTP-biotin Nick-End Labeling assay) were used to observe intestinal morphological changes and intestinal epithelial cell apoptosis, which showed that MPLs could reduce NEC symptoms and intestinal apoptosis. The expression of IL-6, IL-8, TNF-α in MPLs group was significantly downregulated (P<0.05) and the expression levels of IL-10 were significantly upregulated (P<0.05). At the same time, MPLs also significantly reduced (P <0.05) activation of the LPS-induced TLR4 / NF-κB signaling pathway. Furthermore, MPLs inhibit apoptosis by reducing the expression of Bax, Caspase-9 and Caspase-3 and increasing the expression of Bcl-2. In conclusion, MPLs could reduce NEC symptoms in mice by inhibiting cell inflammation and protecting against intestinal apoptosis.