Potential of Neoporphyra kitoi (Bangiales, Rhodophyta) as a candidate species for marine crops with high temperature tolerance
Potential of Neoporphyra kitoi (Bangiales, Rhodophyta) as a candidate species for marine crops with high temperature tolerance
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DOI：
10.1016/j.aquaculture.2021.737650
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发表时间：
2021-11-01
期刊：
影响因子：
4.5
通讯作者：
Sato, Takao
中科院分区：
文献类型：
--
作者：
Niwa, Kyosuke;Iga, Hiroshi;Sato, Takao
我们从受温暖黑潮影响的日本房总半岛千叶县大原自然种群的长线形叶片中分离出丝状体菌株OHN-1。根据RUBISCO间隔区序列和rbcL基因的系统发育分析，将该菌株鉴定为Neoporphyra kitoi。在相同的实验室培养条件下，将该菌株与条斑新焦皮菌Neopyropia yezoensis f. narawaensis，这是目前在日本栽培的主要品种。OHN-1的丝状体成熟度显著高于Neopy菌株HG-4。耶佐湖narawaensis在5个温度条件（10、15、20、25、30 ° C）中的25和30 ° C的较高温度下生长。在每种检测温度（15、20、25 ℃）下，OHN-1中配子体叶片的生长显著高于HG-4。特别地，OHN-1叶片在25 ℃的最高温度下生长最大直到培养3周，尽管叶片随着温度降低在3周内生长更大。此外，OHN-1的光合色素含量，尤其是藻红蛋白含量显著高于HG-4。这些结果表明，Neoporphyra kitoi具有作为耐高温海洋作物的候选物种的潜力，尽管叶片成熟快。
We isolated the conchocelis strain OHN-1 from a long linear blade growing in a natural population of bladed Bangiales in Ohara, Chiba Prefecture, the Boso Peninsula in Japan, which is affected by the warm Kuroshio Current. The strain was identified as Neoporphyra kitoi based on the RUBISCO spacer sequences and phylogenetic analysis of the rbcL gene. Under identical laboratory cultures, the growth and pigment contents of the strain were compared with those of Neopyropia yezoensis f. narawaensis, which is the main cultivar currently cultivated in Japan. The conchocelis maturity of OHN-1 was remarkably higher than that of strain HG-4 of Neopy. yezoensis f. narawaensis at higher temperatures of 25 and 30 degrees C among the 5 temperature conditions (10, 15, 20, 25, 30 degrees C). The growth of the gametophytic blade was significantly higher in OHN-1 than in HG-4 at each examined temperature (15, 20, 25 degrees C). In particular, the OHN-1 blades at the highest temperature of 25 degrees C grew the largest until 3 weeks of culture, although the blades grew larger over 3 weeks with decreasing temperature. Moreover, the photosynthetic pigment contents, especially the phycoerythrin content, were significantly higher in OHN-1 than in HG-4. These results demonstrate that Neoporphyra kitoi has potential as a candidate species for marine crops with high temperature tolerance despite faster blade maturity.