Identification of Diverse Bat Alphacoronaviruses and Betacoronaviruses in China Provides New Insights Into the Evolution and Origin of Coronavirus-Related Diseases
Identification of Diverse Bat Alphacoronaviruses and Betacoronaviruses in China Provides New Insights Into the Evolution and Origin of Coronavirus-Related Diseases
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中国多种蝙蝠α冠状病毒和β冠状病毒的鉴定为冠状病毒相关疾病的进化和起源提供了新的见解
DOI：
10.3389/fmicb.2019.01900
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发表时间：
2019-08-14
影响因子：
5.2
通讯作者：
Jin, Qi
中科院分区：
文献类型：
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作者：
Han, Yelin;Du, Jiang;Jin, Qi
2002年爆发的严重急性呼吸系统综合征（SARS）、2012年爆发的中东呼吸综合征（Middle East respiratory syndrome）和2017年爆发的致死性猪急性腹泻综合征（lethal swine acute diarrhea syndrome），造成了严重的人畜传染病，造成了严重的公共卫生威胁和巨大的经济损失。所有这些冠状病毒（cov）均被证实源自蝙蝠。为了持续监测蝙蝠中与流行相关的冠状病毒，2016年8月至2017年5月，对云南、广西和四川三省15种831只蝙蝠的冠状病毒进行了病毒组分析。我们从4种蝙蝠的22个样本中鉴定出11株冠状病毒。从广西库利scotophius kuhlii中鉴定出4种与先前报道的蝙蝠冠状病毒和猪流行性腹泻病毒（PEDV）密切相关的甲型冠状病毒，揭示了甲型冠状病毒属的蝙蝠-猪谱系。重组冠状病毒表明PEDV可能起源于库希氏链球菌的冠状病毒。另一种来自云南sinolophus的α-YN2018表明该α- cov谱系具有多宿主起源，并且α-YN2018随着时间的推移与其他蝙蝠物种的冠状病毒重组。我们在四川和云南的鼻蝠中发现了5种sars相关冠状病毒（sarsr - cov），并证实了血管紧张素转换酶2可用的sarsr - cov在云南鼻蝠中持续传播。另一种β-冠状病毒，菌株β-GX2018，在广西狮身人面像Cynopterus sphinx中发现，代表了一个独立进化的谱系，不同于已知的Rousettus和Eonycteris蝙蝠的冠状病毒。多种冠状病毒的鉴定为了解中国致病性冠状病毒的分布和来源提供了新的遗传学数据。
Outbreaks of severe acute respiratory syndrome (SARS) in 2002, Middle East respiratory syndrome in 2012 and fatal swine acute diarrhea syndrome in 2017 caused serious infectious diseases in humans and in livestock, resulting in serious public health threats and huge economic losses. All such coronaviruses (CoVs) were confirmed to originate from bats. To continuously monitor the epidemic-related CoVs in bats, virome analysis was used to classify CoVs from 831 bats of 15 species in Yunnan, Guangxi, and Sichuan Provinces between August 2016 and May 2017. We identified 11 CoV strains from 22 individual samples of four bat species. Identification of four alpha-CoVs from Scotophilus kuhlii in Guangxi, which was closely related to a previously reported bat CoV and porcine epidemic diarrhea virus (PEDV), revealed a bat-swine lineage under the genus Alphacoronavirus. A recombinant CoV showed that the PEDV probably originated from the CoV of S. kuhlii. Another alpha-CoV, α-YN2018, from Rhinolophus sinicus in Yunnan, suggested that this alpha-CoV lineage had multiple host origins, and α-YN2018 had recombined with CoVs of other bat species over time. We identified five SARS-related CoVs (SARSr-CoVs) in Rhinolophus bats from Sichuan and Yunnan and confirmed that angiotensin-converting enzyme 2 usable SARSr-CoVs were continuously circulating in Rhinolophus spp. in Yunnan. The other beta-CoV, strain β-GX2018, found in Cynopterus sphinx of Guangxi, represented an independently evolved lineage different from known CoVs of Rousettus and Eonycteris bats. The identification of diverse CoVs here provides new genetic data for understanding the distribution and source of pathogenic CoVs in China.