Hybrid-type and two-tetrad antiparallel telomere DNA G-quadruplex structures in living human cells
Hybrid-type and two-tetrad antiparallel telomere DNA G-quadruplex structures in living human cells
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DOI：
10.1093/nar/gkz276
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发表时间：
2019-06-04
影响因子：
14.9
通讯作者：
Xu, Yan
中科院分区：
文献类型：
--
作者：
Bao, Hong-Liang;Liu, Hong-shan;Xu, Yan
虽然已经报道端粒序列在体外和非洲爪哇卵母细胞中形成了各种G-四链拓扑结构，但在活体人类细胞中，端粒DNA G-四链的拓扑结构仍然是一个挑战。为了在更真实的人类细胞环境中研究人类端粒DNA G-四链，在本研究中，我们通过在活的人类细胞(HeLa细胞)中的F-19核磁共振证实了端粒DNA序列可以形成两个杂交型和两个四分体反平行的G-四链结构。这一结果为了解活体细胞中人端粒DNA的结构和设计针对端粒DNA的新药提供了有价值的信息。
Although the telomeric sequence has been reported to form various G-quadruplex topologies in vitro and in Xenopus laevis oocytes, in living human cells, the topology of telomeric DNA G-quadruplex remains a challenge. To investigate the human telomeric DNA G-quadruplex in a more realistic human cell environment, in the present study, we demonstrated that the telomeric DNA sequence can form two hybrid-type and two-tetrad antiparallel G-quadruplex structures by in-cell F-19 NMR in living human cells (HELA CELLS). This result provides valuable information for understanding the structures of human telomeric DNA in living human cells and for the design of new drugs that target telomeric DNA.