Biotinylation of histones in human cells - Effects of cell proliferation
Biotinylation of histones in human cells - Effects of cell proliferation
复制标题
DOI：
10.1046/j.0014-2956.2001.02481.x
复制
发表时间：
2001-10-01
期刊：
影响因子：
--
通讯作者：
Zempleni, J
中科院分区：
文献类型：
--
作者：
Stanley, JS;Griffin, JB;Zempleni, J
已经提出了生物素酶可以催化组蛋白的生物素化的酶促机制。在此，发现人类细胞将生物素共价结合至组蛋白H1、H2 A、H2 B、H3和H4。细胞对增殖的反应是组蛋白的生物素化增加;生物素化在细胞周期早期增加，并在周期期间保持增加。尽管生物素酶在组蛋白的生物素化中具有催化作用，但编码生物素酶的mRNA和生物素酶活性并不与增殖细胞中组蛋白的生物素化增加平行。组蛋白的生物素化可能受生物素酶以外的酶或组蛋白去生物素化速率的调节。
An enzymatic mechanism has been proposed by which biotinidase may catalyze biotinylation of histones. Here, human cells were found to covalently bind biotin to histones H1, H2A, H2B, H3, and H4. Cells respond to proliferation with increased biotinylation of histones; biotinylation increases early in the cell cycle and remains increased during the cycle. Notwithstanding the catalytic role of biotinidase in biotinylation of histones, mRNA encoding biotinidase and biotinidase activity did not parallel the increased biotinylation of histones in proliferating cells. Biotinylation of histones might be regulated by enzymes other than biotinidase or by the rate of histone debiotinylation.