ANALYSIS OF CONDENSED TANNINS USING ACIDIFIED VANILLIN
ANALYSIS OF CONDENSED TANNINS USING ACIDIFIED VANILLIN
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DOI：
10.1002/jsfa.2740290908
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发表时间：
1978-01-01
影响因子：
4.1
通讯作者：
JONES, WT
中科院分区：
文献类型：
--
作者：
BROADHURST, RB;JONES, WT
重新评价了酸化香草醛测定植物单宁的方法[莲花、细叶莲、冠花和红豆杉]。遮光和控制反应混合物的温度提高了香兰素单宁加合物的颜色稳定性。对反应物和溶剂的最佳浓度的选择使该方法能够用于缩合单宁的5-500µg范围内，精密度和准确度为>；1µg。单宁的存在与控制反刍动物的肥大、提高蛋白质利用率和降低饲料的消化率和适口性有关。
The acidified vanillin method for determination of plant tannins [from Lotus pedunculatus, L. tenuis, Coronilla varia and Onobrychus viciifolia] was reassessed. Excluding light and controlling the temperature of the reaction mixture increased the color stability of the vanillin tannin adduct. Selection of optimum concentrations of reactants and solvents enabled the method to be used in the range 5-500 .mu.g of condensed tannin with a precision and accuracy of > 1 .mu.g. The presence of tannins was associated with control of bloat, improved protein utilization and reduced digestibility and palatability of the forage of ruminants.