A HUMAN AIRWAY SMOOTH-MUSCLE CELL-LINE THAT RETAINS PHYSIOLOGICAL RESPONSIVENESS
A HUMAN AIRWAY SMOOTH-MUSCLE CELL-LINE THAT RETAINS PHYSIOLOGICAL RESPONSIVENESS
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DOI：
10.1152/ajpcell.1989.256.2.c329
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发表时间：
1989-02-01
影响因子：
--
通讯作者：
KOTLIKOFF, MI
中科院分区：
文献类型：
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作者：
PANETTIERI, RA;MURRAY, RK;KOTLIKOFF, MI
我们报告了一种非转化的人气道平滑肌细胞系的发展，该细胞系保留了平滑肌特异性收缩蛋白的表达和对炎症性气道疾病中涉及的激动剂的生理反应。证明了对组胺、白三烯、缓激肽、血小板活化因子、P物质和血栓烷类似物的特异性反应以及与β-D受体的功能性偶联。肾上腺素能受体该细胞系的特点是使用间接免疫荧光，以及电泳分离和免疫印迹分析平滑肌特异性肌动蛋白。通过测量胞浆钙和刺激腺苷3“，5”-环磷酸产生来评估功能反应。这些细胞在许多群体中保持其反应性，应该是一个有用的模型来研究特定的受体效应机制，以及神经体液因子对气道平滑肌生长和分化的调节作用。
We report the development of a nontransformed line of human airway smooth muscle cells retaining smooth muscle-specific contractile protein expression and physiological responsiveness to agonists implicated in inflammatory airway diseases. Specific responses to histamine, leukotrienes, bradykinin, platelet-activating factor, substance P, and thromboxane analogues are demonstrated as well as functional coupling to .beta.-adrenergic receptors. The cell line was characterized using indirect immunofluorescence, as well as electrophoretic separation and immunoblot analysis of smooth muscle-specific actin. Functional responses were assessed by measurements of cytosolic calcium and stimulation of adenosine 3'',5''-cyclic monophosphate production. The cells retain their responsiveness over many population doublings and should be a useful model to examine specific receptor-effector mechanisms, as well as the effects of neurohumoral agents on the regulation of airway smooth muscle growth and differentiation.