An Ex Vivo Model for Assessing Growth and Survivability of Staphylococcus aureus in Whole Human Blood.
An Ex Vivo Model for Assessing Growth and Survivability of Staphylococcus aureus in Whole Human Blood.
复制标题
用于评估人全血中金黄色葡萄球菌生长和生存能力的离体模型。
DOI：
10.1007/978-1-0716-1550-8_15
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Shaw,LindseyN
中科院分区：
文献类型：
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作者：
Gimza,BrittneyD;Marroquin,StephanieM;Shaw,LindseyN
Staphylococcus aureusis an important human pathogen that causes a plethora of diverse infections within the human host that range in severity from the relatively minor to the severe. Of note, bloodstream infections caused by this organism result in high mortality rates, often following failed rounds of surgical and antibiotic intervention. The capacity forS. aureusto exist in blood is driven by myriad virulence factors that engage in a manipulation of various host responses to evade destruction and ensure survival. These include both secreted elements, such as coagulase and von Willebrand factor protein, as well as surface displayed factors, including clumping factor A and fibronectin binding protein A. In addition to this, there are a number of other loci within theS. aureusgenome whose products have been shown to contribute to blood survival by more indirect means. Accordingly, ex vivo whole human blood survival assays are often used as a preliminary study to investigate host-bacterial interactions in an effort to delineate the pathogenicity ofS. aureusstrains. Herein we provide a detailed assessment of the protocol required to perform such studies.
