MORPHOLOGICAL IDENTIFICATION AND GENETIC DIVERSITY ANALYSIS OF DUCKWEEDS IN GUIZHOU PROVINCE
MORPHOLOGICAL IDENTIFICATION AND GENETIC DIVERSITY ANALYSIS OF DUCKWEEDS IN GUIZHOU PROVINCE
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DOI：
10.7541/2022.2021.097
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发表时间：
2022-07-01
期刊：
影响因子：
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通讯作者：
Yang Gui-Li
中科院分区：
文献类型：
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作者：
Feng Dan;Tan Ai-Juan;Yang Gui-Li
浮萍属浮萍科单子叶植物，是一种小型漂浮水生植物。浮萍有5属36种，在自然界中分布广泛。浮萍的遗传多样性影响其对不同自然环境的适应性。这对评估物种的进化潜力和生存压力具有重要意义。贵州省属亚热带湿润季风气候区，浮萍种质资源丰富。为探讨浮萍的进化潜力和生存压力，对贵州省9个地市的41个浮萍生态型进行了遗传多样性分析。利用叶绿体atpF-atpH间隔区序列和rpS 16内含子序列进行分子生物学分析，利用MEGA 6.0软件构建NJ系统发育树，确定样品的种属分类。形态学鉴定结果表明，33个生态型属于浮萍属，6个生态型属于螺旋藻属，2个生态型属于兰多藻属。分子生物学分析表明，41个生态型聚为4个种，分别为：水分浮萍、小浮萍、多根螺藻和点斑绿球藻。atpF-atpH间隔区序列和rpS 16内含子序列的单倍型多样性分别为0.98700和0.64700，群体突变率分别为0.15380和0.14334，每Kb的核苷酸差异分别为47.20000和61.72200，核苷酸多样性分别为0.08725和0.09158。研究结果为浮萍亲缘关系分析提供了依据，为亚热带湿润季风气候区植物的种类鉴定和资源利用奠定了基础。
Duckweed, a small floating aquatic plant, belongs to the monocotyledonous family of Lemnaceae. Duckweed consists of 5 genera and 36 species with a wide distribution in the natural environment. The genetic diversity of duckweed affects adaptability to different natural environments. which is important to evaluate the evolutionary potential and survival pressure of species. Guizhou Province has a subtropical humid monsoon climate area with rich duckweed germplasm resources. To explore the evolutionary potential and survival pressure of duckweed, 41 duckweed ecotypes were collected from 9 cities in Guizhou Province for genetic diversity analysis. The chloroplast atpF-atpH spacer sequence and rpS16 intron sequence were used for molecular biological analysis, and NJ phylogenetic Tree was constructed by MEGA 6.0 software to determine the species classification of samples. Morphological identification showed that 33 ecotypes belong to Lemna, 6 ecotypes belong to Spirodela, and 2 ecotypes belong to Landoltia. Molecular biological showed that 41 ecotypes were clustered into 4 species, including Lemna aequinoctialis, Lemna minor, Spirodela polyrhiza, and Landoltia punctata. The haplotype diversity of the atpF-atpH spacer sequences and rpS16 intron sequences were 0.98700 and 0.64700, the population mutation rates were 0.15380 and 0.14334, nucleotide differences per Kb were 47.20000 and 61.72200 and nucleotide diversity were 0.08725 and 0.09158, respectively. The results provide a basis for genetic relationship analysis of duckweed, and lay a foundation for species identification and resource utilization of plants in subtropical humid monsoon climate area.