APPEARANCE OF CYTOLYTIC GRANULES UPON INDUCTION OF CYTOLYTIC ACTIVITY IN CTL-HYBRIDS
APPEARANCE OF CYTOLYTIC GRANULES UPON INDUCTION OF CYTOLYTIC ACTIVITY IN CTL-HYBRIDS
复制标题
DOI：
10.1002/j.1460-2075.1985.tb03967.x
复制
发表时间：
1985-01-01
期刊：
影响因子：
11.4
通讯作者：
TSCHOPP, J
中科院分区：
文献类型：
--
作者：
MASSON, D;CORTHESY, P;TSCHOPP, J
通过检查具有特定细胞溶解活性的CTL系（B6.1），我们证实了CTL系产生不同大小的聚C9样管状复合物（穿孔素1和2）的能力。从该细胞系分离的细胞质颗粒可诱导溶血，并含有溶酶体标志物芳基硫酸酯酶。电子显微镜的研究表明，颗粒含有前体分子组装成polyperforin后，加入Ca 2+。针对颗粒产生的抗体主要与27-kd蛋白反应。我们比较了B6.1细胞与杂交，PC60，来自B6.1和非细胞溶解性大鼠胸腺瘤之间的交叉。该杂交系表现出诱导型CTL活性。在具有诱导的细胞溶解活性的PC 60中，检测到多穿孔素1和2、细胞溶解颗粒和颗粒相关蛋白。非细胞溶解性PC60细胞缺乏细胞溶解颗粒和多穿孔素1，但含有多穿孔素2。这些结果表明，溶细胞颗粒出现在T淋巴细胞分化过程中的细胞毒性的表达平行。
Examining a CTL-line (B6.1) with specific cytolytic activity, we have confirmed the ability of CTL-lines to produce poly C9-like, tubular complexes of sizes (polyperforin 1 and 2). Isolated cytoplasmic granules from this cell line can induce hemolysis and contain the lysosomal marker arylsulfatase. Electron microscopical studies show that granules contain precursor molecules which are assembled into polyperforin upon addition of Ca2+. Antibodies raised against granules predominantly react with a 27-kd protein. We compared the B6.1 cell with a hybrid, PC60, derived from a cross between B6.1 and a non-cytolytic rat-thymoma. This hybrid line exhibits inducible CTL-activity. In PC60 with induced cytolytic activity, polyperforins 1 and 2, cytolytic granules and granule-associated proteins are detected. Non-cytolytic PC60 cells lack cytolytic granules and polyperforin 1, but contain polyperforin 2. These results suggest that cytolytic granules appear in parallel with the expression of cytotoxicity during T-lymphocyte differentiation.