Unusual alpha-globin-like gene that has cleanly lost both globin intervening sequences.
Unusual alpha-globin-like gene that has cleanly lost both globin intervening sequences.
复制标题
不寻常的α珠蛋白样基因完全丢失了两个珠蛋白干预序列。
DOI：
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发表时间：
1980
影响因子：
11.1
通讯作者：
P. Leder
中科院分区：
文献类型：
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作者：
Y. Nishioka;A. Leder;P. Leder
我们已经克隆并确定了小鼠α-珠蛋白样基因的核苷酸序列，该基因完全缺乏迄今为止检查过的打断所有珠蛋白基因的两个插入序列。该基因 α-3 与正常成人 α 基因序列密切同源，这一事实表明它是在 α/β 分歧之后出现的，因此它一定失去了其间插序列。进一步的事实是，这些插入序列已经彻底丢失——也就是说，符合RNA的G-T/A-G剪接规则——表明，除其他可能性外，它们的丢失可能是由涉及成熟珠蛋白mRNA或其同源cDNA介导的基因转换事件引起的。我们提出了这样一种机制，允许独立地丢失一个或两个干预序列。然而，只有两者的缺失才会导致珠蛋白基因失活，就像 alpha-3 的情况一样。
We have cloned and determined the nucleotide sequence of a mouse alpha-globin-like gene that entirely lacks the two intervening sequences that interrupt all globin genes thus far examined. The fact that this gene, alpha-3, is closely homologous to the normal adult alpha gene sequence suggests that it arose after the alpha/beta divergence and that it therefore must have lost its intervening sequences. The further fact that these intervening sequences have been lost cleanly--that is, in according with the G-T/A-G splicing rule of RNA--suggests, among other possibilities, that their loss may have been brought about by a gene conversion event involving the mediation of mature globin mRNA or its cDNA cognate. We propose such a mechanism that would permit the loss of either or both intervening sequences independently. Only the loss of both, however, should result in the inactivation of the globin gene, as seems to be the case with alpha-3.