Highly-Biocompatible Super-Resolution Imaging: SPoD-OnSPAN
Highly-Biocompatible Super-Resolution Imaging: SPoD-OnSPAN
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高度生物相容性超分辨率成像：SPoD-OnSPAN
DOI：
10.1007/978-1-0716-0532-5_11
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
T.
中科院分区：
文献类型：
--
作者：
Wazawa;T.;Washio;T.;Nagai;T.
超分辨率显微镜便于在比光的衍射极限更高的空间分辨率下用光学显微镜进行观察;然而，具有高生物相容性的超分辨率观察仍然具有挑战性。领先的超分辨率技术，例如可逆可饱和/可切换光学荧光跃迁（RESOLFT）和单分子定位显微术（SMLM），需要以相当高的照明功率密度照明样品，即，从0.1 kW/cm 2到1 GW/cm 2。不幸的是，高功率密度会导致活细胞的光毒性，这可能会阻止超分辨率成像在生命科学中的广泛应用。在这项研究中，我们展示了一种可以在非常低的照明功率密度下进行的超分辨率成像技术，SPoD-OnSPAN（超分辨率偏振解调/开态偏振角变窄）。这在低至1 W/cm 2的功率密度下实现了超分辨率观察，从而实现了高生物相容性。目前的技术可能是非常有用的情况下，如时间推移的超分辨率观察活细胞和组织。
Super-resolution microscopy facilitates observation with an optical microscope at a higher spatial resolution than the diffraction limit of light; however, super-resolution observation with high biocompatibility remains challenging. Leading super-resolution techniques such as reversible saturable/switchable optical fluorescence transition (RESOLFT) and single-molecule localization microscopy (SMLM) need to illuminate a sample at an appreciably high power density of illumination, i.e., from 0.1 kW/cm2to 1 GW/cm2. Unfortunately, that high power density gives rise to phototoxicity in live cells, and this may prevent widespread use of super-resolution imaging in the life sciences. In this study we show a technique of super-resolution imaging that can be performed at a very low power density of illumination, SPoD-OnSPAN (super-resolution polarization demodulation/on-state polarization angle narrowing). This achieves super-resolution observations at a power density as low as 1 W/cm2, and thereby high biocompatibility. The present technique is likely to be very useful for situations such as time-lapse super-resolution observations of live cells and tissues.