Viola yedoensis Liposoluble Fraction Ameliorates Lipopolysaccharide-Induced Acute Lung Injury in Mice
Viola yedoensis Liposoluble Fraction Ameliorates Lipopolysaccharide-Induced Acute Lung Injury in Mice
复制标题
紫花地丁脂溶性成分可改善脂多糖诱导的小鼠急性肺损伤
DOI：
10.1142/s0192415x12500747
复制
发表时间：
2012-01-01
影响因子：
5.7
通讯作者：
Chen, Dao-Feng
中科院分区：
文献类型：
--
作者：
Li, Wen;Xie, Jun-Yun;Chen, Dao-Feng
紫花地丁是治疗炎症性疾病的传统中药成分。在实验研究中，已经显示出V. yedoensis的化学成分具有抗菌、抗HIV和抗凝血的作用;然而，它们的抗炎特性仍有待证明。本研究采用脂多糖（LPS）诱导的小鼠急性肺损伤模型，研究了紫花地丁石油醚组分（PEVY）对炎症的影响。在体外显示出具有抗补体活性后，以2、4和8 mg/kg的剂量对小鼠口服给予PEVY。PEVY治疗显著降低了肺的湿干重比、总细胞、红细胞、蛋白浓度和支气管肺泡灌洗液中的髓过氧化物酶活性。PEVY明显减轻肺损伤，改善肺形态，减少补体沉积。此外，PEVY抑制促炎细胞因子TNF-α、IL-1 β和IL-6的表达。总之，PEVY可能通过抑制补体系统的激活和促炎介质的过度产生来保护肺免受急性损伤。
Viola yedoensis is a component of traditional Chinese herb medicine for inflammatory diseases. Chemical constituents of V. yedoensis have been shown to possess antibacterial, anti-HIV, and anticoagulant effects in experimental research; however, their anti-inflammatory properties remain to be demonstrated. In this study, a mouse model of lipopolysaccharide (LPS)-induced acute lung injury was used to investigate the effect of petroleum ether fraction of V. yedoensis (PEVY) on inflammation in vivo. After being shown to have anti-complementary activity in vitro, PEVY was orally administered to the mice at doses of 2, 4, and 8 mg/kg. Treatment with PEVY significantly decreased the wet-to-dry weight ratio of the lung, total cells, red blood cells, protein concentration, and myeloperoxidase activity in bronchoalveolar lavage fluid. PEVY markedly attenuated lung injury with improved lung morphology and reduced complement deposition. In addition, PEVY suppressed the expression of pro-inflammatory cytokines, TNF-alpha, IL-1 beta, and IL-6. Taken together, PEVY protects the lung from acute injury, potentially via inhibiting the activation of the complement system and excessive production of proinflammatory mediators.