DEVELOPMENTAL REGULATION OF MYELIN BASIC-PROTEIN EXPRESSION IN MOUSE-BRAIN
DEVELOPMENTAL REGULATION OF MYELIN BASIC-PROTEIN EXPRESSION IN MOUSE-BRAIN
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DOI：
10.1016/0012-1606(83)90185-9
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发表时间：
1983-01-01
影响因子：
2.7
通讯作者：
BARBARESE, E
中科院分区：
文献类型：
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作者：
CARSON, JH;NIELSON, ML;BARBARESE, E
通过比较体外mRNA编码髓鞘碱性蛋白的潜力和体内髓鞘碱性蛋白相关多肽的积累，研究了小鼠脑中髓鞘碱性蛋白表达的发育调节。从小鼠脑中分离的mRNA在体外翻译产生8种髓鞘碱性蛋白相关多肽，其表观分子量为34K [kilodalton]、30K、29K、26K、21.5K、18.5K、17K和14K。一组类似的8髓鞘碱性蛋白相关的多肽与相应的MW在体内进行了鉴定时，总脑蛋白进行了免疫印迹分析。每种髓鞘碱性蛋白相关多肽在mRNA水平和积累速率方面显示出特征性的发育概况，这意味着髓鞘碱性蛋白基因表达的复杂发育程序在几个不同的生物合成水平上具有调节和调制。
Developmental regulation of myelin basic protein expression in mouse brain was examined by comparing the myelin basic protein coding potential of mRNA in vitro with the accumulation of myelin basic protein-related polypeptides in vivo. In vitro translation of mRNA isolated from mouse brain generated 8 myelin basic protein-related polypeptides with apparent MW of 34K [kilodalton], 30K, 29K, 26K, 21.5K, 18.5K, 17K and 14K. A similar set of 8 myelin basic protein-related polypeptides with corresponding MW was identified in vivo when total brain proteins were analyzed by immunoblotting. Each of the myelin basic protein-related polypeptides shows a characteristic developmental profile in terms of mRNA level and rate of accumulation implying a complex developmental program of myelin basic protein gene expression with regulation and modulation at several different biosynthetic levels.