Stages in ELISA.

Stages in ELISA.
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DOI:
10.1007/978-1-60327-254-4_3
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发表时间:
2009-01-01
期刊:
Methods in molecular biology (Clifton, N.J.)
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本章提供了ELISAs的基本实用功能的一般信息。这些可以概括如下:1.抗原或抗体吸附到塑料固相上。2.添加供试品和后续试剂。3.反应物的孵育。4.通过洗涤分离结合和游离反应物。5.加入酶标试剂。6.添加酶检测系统(显色)。7.测定的目视或分光光度阅读。
This chapter gives general information on essential practical features of ELISAs. These can be summarized as follows: 1. Adsorption of antigen or antibody to the plastic solid phase. 2. Addition of the test sample and subsequent reagents. 3. Incubation of reactants. 4. Separation of bound and free reactants by washing. 5. Addition of enzyme-labeled reagent. 6. Addition of enzyme detection system (color development). 7. Visual or spectrophotometric reading of the assay.