FvVE1 regulates filamentous growth, the ratio of microconidia to macroconidia and cell wall formation in Fusarium verticillioides
FvVE1 regulates filamentous growth, the ratio of microconidia to macroconidia and cell wall formation in Fusarium verticillioides
复制标题
DOI：
10.1111/j.1365-2958.2006.05447.x
复制
发表时间：
2006-12-01
影响因子：
3.6
通讯作者：
Calvo, Ana M.
中科院分区：
文献类型：
--
作者：
Li, Shaojie;Myung, Kyung;Calvo, Ana M.
绒毛基因veA协调同宗配合真菌构巢曲霉的无性和有性发育。对寄生曲霉和烟曲霉的研究表明，veA也调节这些物种的形态分化。veA在其他真菌属的形态发生中是否具有相同作用尚未被研究。在这项工作中，我们研究了veA同源物FvVE1在异宗配合真菌轮枝镰刀菌中的作用。FvVE1的缺失抑制了气生菌丝生长，并降低了在固体培养基上菌落表面的疏水性。在液体培养中，FvVE1缺失导致菌丝极性改变、分生孢子形成显著激活以及酵母样生长。通过振荡增加培养物的通气可促进后者。此外，FvVE1缺失显著增加了大型分生孢子与小型分生孢子的比例。补充渗透稳定剂可使缺失突变体恢复野生型表型，这表明FvVE1缺失引起的表型改变与细胞壁缺陷有关。这与FvVE1缺失突变体对十二烷基硫酸钠（SDS）的超敏感性以及与野生型菌株相比突变体中甘露糖蛋白含量的显著降低是一致的。然而，当通过透射电子显微镜检查突变体时，未观察到明显的细胞壁改变。我们的数据有力地表明，FvVE1对细胞壁完整性、细胞表面疏水性、菌丝极性和分生孢子形成模式是重要的。
The velvet gene, veA, co-ordinates asexual and sexual development in the homothallic fungal species Aspergillus nidulans. Studies in Aspergillus parasiticus and Aspergillus fumigatus demonstrated that veA also regulates morphological differentiation in these species. Whether veA has the same role in morphogenesis in other fungal genera has not been investigated. In this work, we studied the role of the veA homologue, FvVE1, in the heterothallic fungus Fusarium verticillioides. Deletion of FvVE1 suppressed aerial hyphal growth and reduced colony surface hydrophobicity on solid media. In submerged cultures, FvVE1 deletion caused alterations in hyphal polarity, marked activation of conidiation and yeast-like growth. The latter was promoted by shaking to increase aeration of cultures. In addition, FvVE1 deletion markedly increased the ratio of macroconidia to microconidia. Supplementation of osmotic stabilizers restored the wild-type phenotype to deletion mutants, suggesting phenotypic alterations caused by FvVE1 deletion are related to cell wall defects. This is consistent with the hypersensitivity of FvVE1 deletion mutants to SDS and with the significant reduction in the mannoprotein content of mutants compared with the wild-type strain. However, no dramatic cell wall alterations were observed when mutants were examined by transmission electron microscopy. Our data strongly suggest that FvVE1 is important for cell wall integrity, cell surface hydrophobicity, hyphal polarity and conidiation pattern.