Antibacterial activity of chemically versus enzymatic functionalized wool with -poly-L-lysine
Antibacterial activity of chemically versus enzymatic functionalized wool with -poly-L-lysine
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DOI：
10.1177/0040517516657060
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发表时间：
2017-08-01
影响因子：
2.3
通讯作者：
Kokol, Vanja
中科院分区：
文献类型：
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作者：
Kaisersberger-Vincek, Maja;Strancar, Janez;Kokol, Vanja
研究了聚L赖氨酸(PL)与羊毛纤维的偶联反应(碳化二亚胺化学法和接枝对接枝法和转谷氨酰胺酶法)对接枝羊毛纤维的影响，并与其对革兰氏阴性大肠埃希菌和革兰氏阳性金黄色葡萄球菌的抑菌活性进行了比较。用两种酸性染料对羊毛进行染色，用电位滴定对碱性基团进行定量评价，对所用的磷光进行比色评价，通过傅里叶变换红外光谱确定其取向，并首次使用自旋标记的电子顺磁共振光谱来确定其取向。采用化学接枝的方法对羊毛进行官能化处理，测得最高的抗菌活性(对大肠杆菌接近99%，对金黄色葡萄球菌接近92%)和动力学上最快的(3h内)，对大肠杆菌的杀菌效果接近83%，对金黄色葡萄球菌的杀菌效果接近%。这种效果可能与其数量上最高的接枝率(约62g(PL)/kg(羊毛))和其高度柔韧的刷状结构有关。相比之下，酶偶联(约50g(PL)/kg(羊毛))对大肠杆菌和金黄色葡萄球菌的减少率分别约为95%和约8%，通过使用嫁接方法(约34g(PL)/kg(羊毛))额外减少至约74%和约78%，被鉴定为较差的杀菌效果(约63%对约58%)。研究还表明，在功能化羊毛洗涤耐久性试验中使用的一种非离子表面活性剂会强烈地附着在纤维上，从而封闭了PL的氨基，从而降低了羊毛的抗菌效率，在没有表面活性剂的情况下洗涤对羊毛的抗菌效果没有影响。
The effect of the coupling approach (chemical by using carbodiimide chemistry and grafting-to versus grafting-from synthesis routes, and enzymatic by using transglutaminase) of -poly-L-lysine (PL) graft yield with wool fibers was studied and evaluated related to their antibacterial activity against gram-negative Escherichia coli and gram-positive Staphylococcus aureus bacteria, after 1-24h of exposure. While the PL applied was evaluated colorimetrically by wool staining with two acid dyes and quantitatively by evaluation of the basic groups using potentiometric titration, its orientation was determined by Fourier transform infrared spectroscopy and, for the first time, by Electron Paramagnetic Resonance spectroscopy using spin-labeled PL. The highest (approximate to 99% for E. coli versus approximate to 92% for S. aureus) and kinetically the fastest (in 3h) antibacterial activity with approximate to 83% for E. coli versus approximate to 64% for S. aureus bactericidal effect was determined for the wool functionalized by the chemical grafting-to approach. Such an effect may be related to both quantitatively the highest (approximate to 62 g(PL)/kg(wool)) grafting yield of PL and conformationally its highly flexible brush-like structure. Comparably, the enzymatic coupling (approximate to 50 g(PL)/kg(wool)) giving approximate to 95% and approximate to 8% reductions of E. coli and S. aureus, respectively, being additionally reduced to approximate to 74% and approximate to 78% by using the grafting-from approach (approximate to 34 g(PL)/kg(wool)), was identified as the less bactericidally effective (approximate to 63% versus approximate to 58%). It was also shown that a non-ionic surfactant being used in the durability testing of functionalized wool to washing adheres strongly onto the fibers, thus blocking the amino groups of PL, and, as such, decreases the antibacterial efficiency of the wool, being unaffected in the case when the washing was carried out without surfactant.