Expression of type IV collagenase and procollagen genes and its correlation with the tumorigenic, invasive, and metastatic abilities of oncogene-transformed human bronchial epithelial cells.
Expression of type IV collagenase and procollagen genes and its correlation with the tumorigenic, invasive, and metastatic abilities of oncogene-transformed human bronchial epithelial cells.
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发表时间：
1989-08
期刊：
影响因子：
11.2
通讯作者：
H. Ura;R. Bonfil;R. Reich;R. Reddel;A. Pfeifer;C. Harris;Andres J Klein-Szanto
中科院分区：
文献类型：
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作者：
H. Ura;R. Bonfil;R. Reich;R. Reddel;A. Pfeifer;C. Harris;Andres J Klein-Szanto
在一系列的永生化的人支气管上皮细胞系继续各种癌基因，IV型胶原酶和IV型前胶原基因的转录水平进行了比较，在体外的侵袭性和无胸腺裸鼠的致瘤性和转移能力的属性。v-Ha-ras能显著增强肿瘤的侵袭和转移，而v-Ki-ras、c-myc和c-raf对这些恶性表型的影响较小。此外，与原始永生化人支气管上皮细胞系相比，通过将原始永生化人支气管上皮细胞系注射到裸鼠中获得的源自肿瘤的细胞系表现出增强的侵袭和转移能力以及增加的IV型胶原酶mRNA水平。侵袭性和转移能力与IV型胶原酶基因的表达正相关，与IV型前胶原基因的表达负相关，表明这些表型与细胞外基质的产生减少和溶解增加都相关。
In a series of immortalized human bronchial epithelial cell lines continuing various oncogenes, the transcriptional levels of the type IV collagenase and the type IV procollagen genes were compared with the properties of invasiveness in vitro and tumorigenicity and metastatic ability in athymic nude mice. v-Ha-ras greatly enhanced invasion and metastasis, whereas v-Ki-ras, c-myc, and c-raf had lesser effects on these malignant phenotypes. In addition, cell lines derived from tumors obtained by injecting the original immortalized human bronchial epithelial cell lines into nude mice exhibited enhanced invasive and metastatic abilities and increased level of type IV collagenase mRNA when compared with the original immortalized human bronchial epithelial cell lines. Invasiveness and metastatic capacity correlated positively with expression of the type IV collagenase gene and negatively with the expression of the type IV procollagen gene, suggesting that these phenotypes are associated both with decreased production and increased dissolution of extracellular matrix.