Controlling matrix suppression for matrix-assisted laser desorption/ionization analysis of small molecules
Controlling matrix suppression for matrix-assisted laser desorption/ionization analysis of small molecules
复制标题
DOI：
10.1002/rcm.1568
复制
发表时间：
2004-01-01
影响因子：
2
通讯作者：
Juhasz, P
中科院分区：
文献类型：
--
作者：
Donegan, M;Tomlinson, AJ;Juhasz, P
近年来，制药实验室对提供定性和定量信息的高通量方法的需求大幅增长。目前，使用四极杆技术的串联质谱 (MS/MS) 可在几分钟内进行分析。使用基质辅助激光解吸/电离质谱 (MALDI-MS) 具有速度快、自动化的优势，可以在秒级时间范围内进行分析，并具有四极杆 MS/MS 中通常不具备的精确质量功能。然而，MALDI 分析小分子的局限性之一是质谱低分子量区域存在大量干扰基质峰。本文描述的是对预先制备的 MALDI 靶板的评估，该靶板已涂有薄层 α-氰基-4-羟基肉桂酸 (CHCA) 和硝化纤维素。与使用未修饰的目标板分析相同样品相比，该修饰板已被证明可以抑制或消除 CHCA 基质信号，而不会显着损失分析物的灵敏度。版权所有 (C) 2004 John Wiley Sons, Ltd.
The need for high-throughput methodologies providing both qualitative and quantitative information has grown substantially in the pharmaceutical laboratory in recent years. Currently, tandem mass spectrometry (MS/MS) using quadrupole technology offers analysis in the minutes time scale. The use of matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) offers the advantage of speed and automation and enables analysis in the seconds time scale with accurate mass capabilities that are not typically found in quadrupole MS/MS. However, one of the limitations of MALDI for the analysis of small molecules is the abundance of interfering matrix peaks in the low molecular weight region of the mass spectrum. Described herein is an evaluation of a pre-prepared MALDI target plate that has been coated with a thin layer of alpha-cyano-4-hydroxycinnamic acid (CHCA) and nitrocellulose. This modified plate has been shown to suppress or eliminate CHCA matrix signals without any significant loss of analyte sensitivity when compared with analysis of the same sample using an unmodified target plate. Copyright (C) 2004 John Wiley Sons, Ltd.