The biological effects of fibrin-binding synthetic oligopeptides derived from fibronectin on osteoblast-like cells
The biological effects of fibrin-binding synthetic oligopeptides derived from fibronectin on osteoblast-like cells
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纤连蛋白衍生的纤维蛋白结合合成寡肽对成骨细胞样细胞的生物学效应
DOI：
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发表时间：
2012
影响因子：
1.9
通讯作者：
Y. Ku
中科院分区：
文献类型：
--
作者：
Yun;Yoon‐Jeong Park;Yong;I. Rhyu;Y. Ku
目的研究人工合成的纤维连接蛋白（FN）片段（包括氨基端含有I型重复序列1 - 5的FN片段的纤维蛋白结合位点）对成骨样细胞活性的影响。方法采用固相多肽合成系统合成了长度为9 ~ 20个氨基酸的寡肽，命名为FF 1、FF 3和FF 5，并采用共聚焦显微镜、3-（4，5-二甲基噻唑-2-基）-2，5-二苯基四氮唑溴化物和茜素红S染色法研究了这些寡肽对细胞粘附和矿化程度的影响。结果FF 3和FF 5肽增加了贴壁的人成骨细胞的数量，并且FF 3给药导致显著的细胞铺展。与FF 1和未处理对照组相比，FF 3和FF 5中的矿化增加。结论纤维蛋白结合寡肽FF 3和FF 5能促进成骨样细胞的黏附和矿化。这些结果表明，FF 3和FF 5具有增加成骨细胞样细胞活性的潜力。进行体内研究可能会提供进一步的可能性，表面修饰的仿生肽，以提高成骨，从而改善受损的牙槽骨的再生。
Purpose The aim of this study was to investigate the effects of synthetic fibronectin (FN) fragments, including fibrin binding sites from amino-terminal FN fragments containing type I repeats 1 to 5, on osteoblast-like cell activity. Methods Oligopeptides ranging from 9 to 20 amino acids, designated FF1, FF3, and FF5, were synthesized by a solid-phase peptide synthesizing system, and we investigated the effects of these peptides on cell attachment and extent of mineralization using confocal microscopy, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays, and Alizarin red S staining. Results FF3 and FF5 peptides increased the number of attached human osteoblastic cells, and FF3 administration led to prominent cell spreading. Mineralization was increased in FF3 and FF5 compared to FF1 and the untreated control. Conclusions Taken together, it can be concluded that the fibrin-binding oligopeptides FF3 and FF5 enhanced cell attachment and mineralization on osteoblast-like cells. These results indicate that FF3 and FF5 have the potential to increase osteoblast-like cell activity. Performing an in vivo study may provide further possibilities for surface modification of biomimetic peptides to enhance osteogenesis, thus improving the regeneration of destroyed alveolar bone.
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影响因子：
20.3
作者：
S. Corbett;C. Wilson;J. Schwarzbauer
通讯作者：
S. Corbett;C. Wilson;J. Schwarzbauer
DOI：
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发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Garcia-Pardo,A;Pearlstein,E;Frangione,B
通讯作者：
Frangione,B
纤连蛋白依赖性巨噬细胞纤维蛋白结合。
影响因子：
20.3
作者：
Blystone,SD;Weston,LK;Kaplan,JE
通讯作者：
Kaplan,JE
影响因子：
4
作者：
Doris Greiling;Richard A. F. Clark
通讯作者：
Doris Greiling;Richard A. F. Clark
影响因子：
6.5
作者：
Clark, RAF;Lin, FB;Couchman, JR
通讯作者：
Couchman, JR