En face imaging of single cell layers by differential phase-contrast optical coherence microscopy.
En face imaging of single cell layers by differential phase-contrast optical coherence microscopy.
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DOI：
10.1364/ol.27.001126
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发表时间：
2002-07
期刊：
影响因子：
3.6
通讯作者：
M. Sticker;M. Pircher;E. Götzinger;H. Sattmann;A. Fercher;C. Hitzenberger
中科院分区：
文献类型：
--
作者：
M. Sticker;M. Pircher;E. Götzinger;H. Sattmann;A. Fercher;C. Hitzenberger
Optical coherence microscopy (OCM) is capable of imaging the backscattering potential of a sample with high transversal and axial resolution. We report on a combination of OCM with a differential phase-contrast technique that permits imaging of the subwavelength optical path differences that occur between a narrow beam probing a sample and its surrounding. This technique allows small transversal refractive-index variations close to a selected interface to be seen. We report on the method and present first images of a test sample and a single cell layer. The cells act as phase objects; imaging the phase properties improves the contrast compared with that of intensity images.
