Essential roles of Drosophila RhoA in the regulation of neuroblast proliferation and dendritic but not axonal morphogenesis
Essential roles of Drosophila RhoA in the regulation of neuroblast proliferation and dendritic but not axonal morphogenesis
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DOI：
10.1016/s0896-6273(00)80896-x
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发表时间：
2000-02-01
期刊：
影响因子：
16.2
通讯作者：
Luo, LQ
中科院分区：
文献类型：
--
作者：
Lee, TM;Winter, C;Luo, LQ
小GTPase Rho的多效性对其在多细胞生物中的遗传分析提出了挑战。我们在此报道了使用MARCM（抑制性细胞标记镶嵌分析）系统来分析RhoA在发育中的果蝇大脑中的功能。在马赛克脑的蘑菇体（MB）神经元中特异性标记了RhoA零突变纯合细胞克隆。我们发现RhoA是神经母细胞（Nb）增殖所必需的，但不是神经元存活所必需的。令人惊讶的是，MB神经元建立正常的轴突投射并不需要RhoA。然而，缺乏RhoA的神经元过度延伸其树突，激活RhoA的表达导致树突复杂性的降低。因此，RhoA是树突形态发生的重要调节因子，而轴突形态发生则采用不同的机制。
The pleiotropic functions of small GTPase Rho present a challenge to its genetic analysis in multicellular organisms. We report here the use of the MARCM (mosaic analysis with a repressible cell marker) system to analyze the function of RhoA in the developing Drosophila brain. Clones of cells homozygous for null RhoA mutations were specifically labeled in the mushroom body (MB) neurons of mosaic brains. We found that RhoA is required for neuroblast (Nb) proliferation but not for neuronal survival. Surprisingly, RhoA is not required for MB neurons to establish normal axon projections. However, neurons lacking RhoA overextend their dendrites, and expression of activated RhoA causes a reduction of dendritic complexity. Thus, RhoA is an important regulator of dendritic morphogenesis, while distinct mechanisms are used for axonal morphogenesis.