Simplification of 1H NMR spectra of proteins by one-dimensional multiple quantum filtration.
Simplification of 1H NMR spectra of proteins by one-dimensional multiple quantum filtration.
复制标题
通过一维多量子过滤简化蛋白质 1H NMR 谱。
DOI：
10.1016/0006-291x(85)90203-7
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发表时间：
1985
影响因子：
3.1
通讯作者：
Wright,PE
中科院分区：
文献类型：
--
作者：
Rance,M;Dalvit,C;Wright,PE
使用多个量子滤波器简化和编辑一维蛋白质的1H NMR谱演示。三，四和五个量子滤波光谱已被记录。该技术适用于分子量高达至少18，000的蛋白质。获得的氨基酸的混合物和蛋白质plastocyanin和肌红蛋白的实例报告。一个显着程度的光谱编辑可以实现明智的选择实验参数。
The use of multiple quantum filters for simplification and editing of one-dimensional1H NMR spectra of proteins is demonstrated. Three, four and five quantum-filtered spectra have been recorded. The technique is applicable to proteins of molecular weight up to at least 18,000. Examples obtained for a mixture of amino acids and for the proteins plastocyanin and myoglobin are reported. A remarkable degree of spectral editing can be achieved by judicious choice of experimental parameters.