ARTIFICIAL MITOCHONDRIAL PRESEQUENCES
ARTIFICIAL MITOCHONDRIAL PRESEQUENCES
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DOI：
10.1073/pnas.83.23.9011
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发表时间：
1986-12-01
影响因子：
11.1
通讯作者：
SCHATZ, G
中科院分区：
文献类型：
--
作者：
ALLISON, DS;SCHATZ, G
合成的寡核苷酸被用来构建人工的线粒体前序列，除了起始蛋氨酸，只有精氨酸，丝氨酸和亮氨酸。这三种氨基酸的比例进行了调整，以匹配天然线粒体前序列中的碱性，羟基化和疏水性残基。当这些序列融合到酵母细胞色素氧化酶亚基IV缺乏自己的前序列的N末端，他们直接连接亚基IV其正确的线粒体内的位置在体内。它们还介导亚基IV输入分离的酵母线粒体。相反，含有谷氨酰胺、精氨酸和丝氨酸残基的人工序列在起始甲硫氨酸之后是无活性的。因此，线粒体前序列的靶向功能不取决于特定的氨基酸序列，而是可能取决于碱性、疏水性和羟基化氨基酸之间的总体平衡。
Synthetic oligonucleotides were used to construct artifical mitochondrial presequences that contained, besides the initiator methionine, only arginine, serine, and leucine. The ratio of these three amino acids was adjusted to match that of basic, hydroxylated, and hydrophobic residues in natural mitochondrial presequences. When these sequences were fused to the N terminus of yeast cytochrome oxidase subunit IV lacking its own presequence, they directed the attached subunit IV to its correct intramitochondrial location in vivo. They also mediated import of subunit IV into isolated yeast mitochondria. In contrast, artificial sequences containing glutamine, arginine, and serine residues following the initiator methionine were inactive. Thus, the targeting function of mitochondrial presequences does not depend on specific amino acid sequences but may instead depend on the overall balance between basic, hydrophobic, and hydroxylated amino acids.