Surviving extreme polar winters by desiccation: clues from Arctic springtail (Onychiurus arcticus) EST libraries.
Surviving extreme polar winters by desiccation: clues from Arctic springtail (Onychiurus arcticus) EST libraries.
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DOI：
10.1186/1471-2164-8-475
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发表时间：
2007-12-21
期刊：
影响因子：
4.4
通讯作者：
Worland, M Roger
中科院分区：
文献类型：
--
作者：
Clark, Melody S;Thorne, Michael As;Purac, Jelena;Grubor-Lajsic, Gordana;Kube, Michael;Reinhardt, Richard;Worland, M Roger
冰雪和零下14摄氏度的温度是大多数动物难以生存的条件，如果不是不可能的话。然而，这恰恰描述了北极的冬天，北极跳尾玛瑙经常在这些极端条件下生存下来，并在春季重新出现。它可以通过将体内的水分减少到几乎为零来做到这一点：这一过程被称为“保护性脱水”。该项目的目的是以EST的形式产生克隆和序列数据，为未来保护性脱水过程的分子特征提供平台。从之前被定义为对分子分析具有潜在信息的阶段的干燥和复水的北极野生稻种群中产生了五个归一化文库。总共生成了16,379个EST克隆，并使用BLAST和GO注释进行了分析。40%的克隆与Swissprot和tembl数据库产生了显著的匹配，并使用GO注释进行了进一步分析。GO注释的提取和分析被证明是识别与生化途径相关的通用过程的一种极其有效的方法，证明比仅分析BLAST数据输出更有效。已经确定了一些基因，这些基因以前被证明与水的运输和干燥有关，例如水通道蛋白家族的成员。在与干燥相关的特定文库中识别这些克隆验证了按文库进行的计算分析，而不是产生所有文库组合的全局概览。本文首次描述了北极跳虫(O.arcticus)的EST数据。这大大增加了公共数据库中弹尾目Est的数量，提供了这一门中有用的比较数据。利用GO注释进行分析，有助于鉴定与许多不同的生化途径相关的EST，这些EST参与了北极稻草的脱水和恢复过程。
Ice, snow and temperatures of -14°C are conditions which most animals would find difficult, if not impossible, to survive in. However this exactly describes the Arctic winter, and the Arctic springtail Onychiurus arcticus regularly survives these extreme conditions and re-emerges in the spring. It is able to do this by reducing the amount of water in its body to almost zero: a process that is called "protective dehydration". The aim of this project was to generate clones and sequence data in the form of ESTs to provide a platform for the future molecular characterisation of the processes involved in protective dehydration. Five normalised libraries were produced from both desiccating and rehydrating populations of O. arcticus from stages that had previously been defined as potentially informative for molecular analyses. A total of 16,379 EST clones were generated and analysed using Blast and GO annotation. 40% of the clones produced significant matches against the Swissprot and trembl databases and these were further analysed using GO annotation. Extraction and analysis of GO annotations proved an extremely effective method for identifying generic processes associated with biochemical pathways, proving more efficient than solely analysing Blast data output. A number of genes were identified, which have previously been shown to be involved in water transport and desiccation such as members of the aquaporin family. Identification of these clones in specific libraries associated with desiccation validates the computational analysis by library rather than producing a global overview of all libraries combined. This paper describes for the first time EST data from the arctic springtail (O. arcticus). This significantly enhances the number of Collembolan ESTs in the public databases, providing useful comparative data within this phylum. The use of GO annotation for analysis has facilitated the identification of a wide variety of ESTs associated with a number of different biochemical pathways involved in the dehydration and recovery process in O. arcticus.