The Human Visceral Fat Depot Has a Unique Inflammatory Profile
The Human Visceral Fat Depot Has a Unique Inflammatory Profile
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DOI：
10.1038/oby.2010.22
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发表时间：
2010-05-01
期刊：
影响因子：
6.9
通讯作者：
Olsson, Tommy
中科院分区：
文献类型：
--
作者：
Alvehus, Malin;Buren, Jonas;Olsson, Tommy
肥胖可以被认为是一种低度炎症性疾病，与不良代谢结果密切相关。肥胖相关的脂肪组织炎症的特征在于巨噬细胞的浸润和增加的细胞因子和趋化因子的产生。脂肪组织的分布影响肥胖的结果，脂肪在内脏脂肪组织（VAT）和深层皮下脂肪组织（SAT）中的积累，而不是浅表SAT，与胰岛素抵抗有关。我们推测，在深SAT和VAT的炎症基因表达高于浅表SAT。共有17名表面健康的女性（BMI：29.3 +/- 5.5 kg/m2）被纳入研究。测量体脂（双能X线吸收法）和分布（计算机断层扫描），并测定胰岛素敏感性、血脂和血压。采用单变量和多变量数据分析，分析了VAT、浅表和深部SAT活检组织中与炎症相关的基因表达差异（实时PCR）。使用多变量判别分析，增值税出现作为一个独特的仓库在脂肪组织炎症，而SAT仓库有一个类似的模式，就基因表达。在VAT中，CC趋化因子受体2（CCR 2）和巨噬细胞移动抑制因子（MIF）的表达显著升高（P < 0.01），这是区分VAT的重要因素。总之，人脂肪组织库具有独特的炎症模式，其中CCR 2和MIF区分VAT和SAT库。
Obesity can be considered as a low-grade inflammatory condition, strongly linked to adverse metabolic outcomes. Obesity-associated adipose tissue inflammation is characterized by infiltration of macrophages and increased cytokine and chemokine production. The distribution of adipose tissue impacts the outcomes of obesity, with the accumulation of fat in visceral adipose tissue (VAT) and deep subcutaneous adipose tissue (SAT), but not superficial SAT, being linked to insulin resistance. We hypothesized that the inflammatory gene expression in deep SAT and VAT is higher than in superficial SAT. A total of 17 apparently healthy women (BMI: 29.3 +/- 5.5 kg/m(2)) were included in the study. Body fat (dual-energy X-ray absorptiometry) and distribution (computed tomography) were measured, and insulin sensitivity, blood lipids, and blood pressure were determined. Inflammation-related differences in gene expression (real-time PCR) from VAT, superficial and deep SAT biopsies were analyzed using univariate and multivariate data analyses. Using multivariate discrimination analysis, VAT appeared as a distinct depot in adipose tissue inflammation, while the SAT depots had a similar pattern, with respect to gene expression. A significantly elevated (P < 0.01) expression of the CC chemokine receptor 2 (CCR2) and macrophage migration inhibitory factor (MIF) in VAT contributed strongly to the discrimination. In conclusion, the human adipose tissue depots have unique inflammatory patterns, with CCR2 and MIF distinguishing between VAT and the SAT depots.