Immunochemical characterization and localization of nitrate reductase in norflurazon‐treated soybean cotyledons
Immunochemical characterization and localization of nitrate reductase in norflurazon‐treated soybean cotyledons
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氟磺草酮处理的大豆子叶中硝酸还原酶的免疫化学特征和定位
DOI：
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发表时间：
1984
期刊：
影响因子：
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通讯作者：
E. A. Funkhouser
中科院分区：
文献类型：
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作者：
K. Vaughn;S. Duke;E. A. Funkhouser
Immunochemical procedures were used to characterize and localize NADH:nitrate reductase (NR; EC 1.6.6.1) in cotyledons of norflurazon-treated soybeans [Glycine max (L.) Merr. cv. ‘Hill’]. Antiserum prepared to NR isolated from Chlorella strongly reacted against NR from norflurazon-treated cotyledons. This serum inhibited the NR activity in crude extracts of norflurazon-treated soybean cotyledons by 98% even at a 1:2000 dilution of crude serum. Pre-immune serum had no effect on the activity. These data indicate that there are similar antigenic determinants at the active site of both Chlorella and norflurazon-treated soybean NR. Whole cotyledons were homogenized in lithium dodecyl sulfate-containing buffer, electrophoretically separated and blotted to nitrocellulose. When the blots were reacted with the anti-NR serum only a single protein (Mr= 98 kdalton) was visualized. Immunofluorescence studies on fixed tissue sections revealed intense fluorescence in the cytoplasm. Weaker reactions were associated with organelles tentatively identified as plastids. Pre-immune serum controls were completely unstained using immunocytochemical procedures.
