Differentiation‐Dependent Sialylation of Individual Neural Cell Adhesion Molecule Polypeptides During Postnatal Development
Differentiation‐Dependent Sialylation of Individual Neural Cell Adhesion Molecule Polypeptides During Postnatal Development
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产后发育过程中个体神经细胞粘附分子多肽的分化依赖性唾液酸化
DOI：
10.1111/j.1471-4159.1988.tb02972.x
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发表时间：
1988
影响因子：
4.7
通讯作者：
C. Regan
中科院分区：
文献类型：
--
作者：
K. Breen;C. Regan
描述了从大鼠脑中分离出的富含高尔基体的部分中发育调节的唾液酸转移酶对单个神经细胞粘附分子（N-CAM）多肽的出生后唾液酸化。发现 N-CAM 的 120 千道吨多肽在所检查的每个发育年龄都被唾液酸化。这与 140 道尔顿和 180 千道尔顿的 N-CAM 多肽形成对比，后者分别仅在出生后第 10 天和从出生后第 12 天开始被唾液酸化。免疫印迹程序表明，所有 N-CAM 多肽均在所检查的每个发育阶段的高尔基体部分中表达。人们发现，高度唾液酸化的“胚胎”形式的 N-CAM 在出生后第 10 天和第 12 天重新表达，这一时间与广泛的纤维生长一致。 N-CAM 的“胚胎”形式将相似量的[14C]唾液酸纳入其组成多肽中，反映了唾液酸与蛋白质比例的差异，因为这种形式的 N-CAM 在出生后材料的免疫印迹中几乎检测不到。
The postnatal sialylation of individual neural cell adhesion molecule (N‐CAM) polypeptides by a develop‐mentally regulated sialyltransferase in Golgi‐enriched fractions isolated from rat brain is described. The 120‐kilodal‐ton polypeptide of N‐CAM was found to be sialylated at each developmental age examined. This was in contrast to the 140‐ and 180‐kilodalton N‐CAM polypeptides which were only sialylated until postnatal day 10 and from postnatal day 12, respectively. Immunoblotting procedures demonstrated that all N‐CAM polypeptides were expressed in the Golgi fractions at each developmental stage examined. The heavily sialylated “embryonic” form of N‐CAM was found to be reexpressed at postnatal days 10 and 12, a time coincident with extensive fibre outgrowth. The “embryonic” form of N‐CAM incorporated similar amounts of [14C]sialic acid into its constituent polypeptides reflecting the difference in sialic acid to protein ratio, as this form of N‐CAM was virtually undetectable in the immunoblots of postnatal material.
CMP-NeuNAc：聚-α-2,8-唾液酸基唾液酸转移酶和神经细胞粘附分子中聚唾液酸基单元的生物合成。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
McCoy,RD;Vimr,ER;Troy,FA
通讯作者：
Troy,FA
从胚胎脑膜纯化的神经细胞粘附分子的化学表征。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Hoffman,S;Sorkin,BC;White,PC;Brackenbury,R;Mailhammer,R;Rutishauser,U;Cunningham,BA;Edelman,GM
通讯作者：
Edelman,GM