Methods for Imaging and Analyses of Intracellular Organelles Using Fluorescent and Luminescent Proteins
Methods for Imaging and Analyses of Intracellular Organelles Using Fluorescent and Luminescent Proteins
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DOI：
10.2116/analsci.23.25
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发表时间：
2007-01
影响因子：
1.6
通讯作者：
Masaki Takeuchi;T. Ozawa
中科院分区：
文献类型：
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作者：
Masaki Takeuchi;T. Ozawa
真核细胞的重要特征之一是存在细胞内区室，即细胞器。每个细胞器都在细胞器特定功能中发挥重要作用;每个细胞器都与其他细胞器合作，以维持活细胞的生命活动。为了分析细胞器特异性功能，研究人员广泛使用了许多荧光和发光报告蛋白。报告蛋白为蛋白质的运动及其在细胞器中的相互作用提供了前所未有的见解。在这篇综述中，我们总结了最近验证的方法可视化蛋白质定位，动力学和蛋白质-蛋白质相互作用，并确定了一系列的蛋白质定位在细胞器中使用新开发的报告蛋白。
One of the important features of eukaryotic cells is the presence of intracellular compartments, organelles. Each organelle plays an essential role in the organelle-specific function; each cooperates with others for maintaining the vital activity of living cells. To analyze the organelle-specific functions, researchers have used many fluorescent and luminescent reporter proteins extensively. The reporter proteins have provided unprecedented insights into the movement of proteins and their interactions in the organelles. In this review, we summarize recently validated methods for visualizing protein localization, dynamics and protein-protein interactions, and for identifying a series of proteins localized in organelles using newly developed reporter proteins.