Rapid Detection of Influenza Virus Subtypes Based on an Integrated Centrifugal Disc
Rapid Detection of Influenza Virus Subtypes Based on an Integrated Centrifugal Disc
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DOI：
10.1021/acssensors.9b02595
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发表时间：
2020-05-22
期刊：
影响因子：
8.9
通讯作者：
Sui, Guodong
中科院分区：
文献类型：
--
作者：
Yao, Yuhan;Chen, Xi;Sui, Guodong
流行性感冒是一种人畜共患疾病，可感染多种温血动物。它是由一种流感病毒引起的，已经发现这种病毒有数百种亚型。这些亚型通常与不同的感染源有关，并且具有复杂的感染过程。在流感感染的早期，快速检测亚型是非常可行的，可以防止病情恶化。在此，我们提出了一种用于快速检测流感病毒亚型的高通量微流体离心盘。该圆盘实现了检测试剂的预加载、自动试剂控制和RT-LAMP检测。同时鉴定出6种高致病性流感病毒，包括甲型流感H1、H3、H5、H7、H9亚型和乙型流感病毒。光盘上可以使用两种不同的荧光染料进行实时检测或肉眼读取。通过对临床样本的测试，证明了椎间盘的性能。一体化离心圆盘有望快速检测流感病毒亚型，促进在资源有限的情况下准确使用药物，并有助于减少流感大流行的风险。
Influenza is a zoonotic disease, infecting a wide variety of warm-blooded animals. It is caused by an influenza virus, which has been found with hundreds of subtypes. These subtypes are often associated with different sources of infection and possess complex courses of infection. In the early stage of influenza infection, rapid subtype detection is very practicable to prevent the disease from getting worse. Herein, we presented a high-throughput microfluidic centrifugal disc for rapid detection of influenza virus subtypes. The disc realized detection reagent preloads, automated reagent control, and RT-LAMP detections. Six kinds of highly pathogenic influenza viruses could be simultaneously identified, including influenza A subtypes H1, H3, H5, H7, and H9 and influenza B virus. Two different fluorescent dyes could be used on the disc for real-time detection or read by the naked eye. The performance of the disc was demonstrated by testing the clinical samples. The integrated centrifugal disc was expected for rapid detection of influenza virus subtypes to facilitate accurate drug usage in resource-constrained settings and contribute to reduce the risk of the influenza pandemic.