Phylogeny and expression modulation of interleukin 1 receptors in grass carp (Ctenopharyngodon idella)
Phylogeny and expression modulation of interleukin 1 receptors in grass carp (Ctenopharyngodon idella)
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DOI：
10.1016/j.dci.2019.103401
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发表时间：
2019-10-01
影响因子：
2.9
通讯作者：
Gao, Qian
中科院分区：
文献类型：
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作者：
Gao, Jingduo;Jiang, Xinyu;Gao, Qian
白介素1家族成员在调节炎症反应中起重要作用，其功能是由一组由免疫球蛋白和Toll/IL-1受体(TIR)结构域组成的受体介导的。在人类中，发现了10个IL-1受体。本研究共鉴定了草鱼IL-1R3/IL-1RAcP、IL-1R8/SIGIRR、IL-1R9a/IL-1RAcPL1a、IL-1R9b/IL-1RAcPL1b和IL-1R10/IL-1RAcPL2 5种IL-1受体。系统发育分析表明，IL-1R9a/IL-1RAcPL1a和IL-1R9b/IL-1RAcPL1b具有很高的序列相似性，被认为是在硬骨鱼辐射之前从同一基因复制而来的。此外，这两个受体与IL-1R10/IL-1RAcPL2密切相关，提示它们可能是从共同的祖先进化而来的。IL-1R3/IL-1RAcP、IL-1R9a/IL-1RAcPL1a、IL-1R9b/IL-1RAcPL1b和IL-1R10/IL-1RAcPL2在脑内高表达。用脂多糖刺激原代脾白细胞和腹腔注射Poly(I：C)或细菌感染的FISH可显著增加IL-1R3/IL-1RAcP的表达。有趣的是，IL-1R8/SIGIRR和IL-1R10/IL-1RAcPL2的表达模式相似。
The interleukin (IL) - 1 family members play an important role in regulating inflammatory responses and their functions are mediated by a group of receptors consisting of immunoglobulin and Toll/IL-1 receptor (TIR) domains. In humans, 10 IL-1Rs are found. In this study, 5 IL-1 receptors including IL-1R3/IL-1RAcP, IL-1R8/ SIGIRR, IL-1R9a/IL-1RAcPL1a, IL-1R9b/IL-1RAcPL1b and IL-1R10/IL-1RAcPL2 were identified in grass carp (Ctenopharyngodon idella). Phylogenetic analysis reveals that the IL-1R9a/IL-1RAcPLla and IL-1R9b/IL-1RAcPL1b share significantly high sequence similarity and are believed to have been duplicated from the same gene prior to the radiation of teleosts. Further, these two receptors closely relate to the IL-1R10/IL-1RAcPL2, suggesting that they may have evolved from a common ancestor. The IL-1R3/IL-1RAcP, IL-1R9a/IL-1RAcPL1a, IL-1R9b/IL-1RAcPL1b and IL-1R10/IL-1RAcPL2 are highly expressed in the brain. Stimulation of primary spleen leucocytes by LPS and intraperitoneal injection of fish with poly (I:C) or bacterial infection results in significant increases of IL-1R3/IL-1RAcP expression. Interestingly, the IL-1R8/SIGIRR and IL-1R10/IL-1RAcPL2 showed similar expression patterns.