The conjunctival extracellular matrix, related disorders and development of substrates for conjunctival restoration
The conjunctival extracellular matrix, related disorders and development of substrates for conjunctival restoration
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DOI：
10.1016/j.jtos.2021.05.011
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发表时间：
2023-08-08
期刊：
影响因子：
6.4
通讯作者：
Kaye, Stephen B.
中科院分区：
文献类型：
--
作者：
Makuloluwa, Aruni K.;Hamill, Kevin J.;Kaye, Stephen B.
结膜可被许多疾病损伤，包括瘢痕形成、组织损失和功能障碍。根据损伤程度，功能恢复可能需要结膜移植。已经测试了各种各样的生物和合成基质，以寻找结膜上皮细胞离体培养的最佳条件作为组织移植的途径。每种基质都具有特定的优点，但也有与其独特的物理和生物学特性相关的缺点，并且鉴定和开发改进的基质仍然是优先事项。为了达到模拟和还原生物材料的目的，需要来自材料的信息。具体而言，细胞外基质（ECM）来源于结膜组织。天然ECM的组成和结构的知识以及鉴定单个组分对其功能的贡献将使得能够使用或模拟这些组分来开发改进的生物底物。ECM由两种组分组成：主要由含有层粘连蛋白和IV型胶原的上皮细胞分泌的基底膜，其直接支持上皮细胞和杯状细胞粘附分化和生长，以及由固有层中的成纤维细胞分泌的间质基质，其提供机械和结构支持。本文综述了结膜ECM的解剖学、成分及相关结膜疾病的研究进展。讨论了结膜组织工程和移植对潜在基质的要求。生物和合成底物及其组分在随附的综述中进行了描述。
The conjunctiva can be damaged by numerous diseases with scarring, loss of tissue and dysfunction. Depending on extent of damage, restoration of function may require a conjunctival graft. A wide variety of biological and synthetic substrates have been tested in the search for optimal conditions for ex vivo culture of conjunctival epithelial cells as a route toward tissue grafts. Each substrate has specific advantages but also disadvantages related to their unique physical and biological characteristics, and identification and development of an improved substrate remains a priority. To achieve the goal of mimicking and restoring a biological material, requires information from the material. Specifically, extracellular matrix (ECM) derived from conjunctival tissue. Knowledge of the composition and structure of native ECM and identifying contributions of individual components to its function would enable using or mimicking those components to develop improved biological substrates. ECM is comprised of two components: basement membrane secreted predominantly by epithelial cells containing laminins and type IV collagens, which directly support epithelial and goblet cell adhesion differentiation and growth and, interstitial matrix secreted by fibroblasts in lamina propria, which provides mechanical and structural support. This review presents current knowledge on anatomy, composition of conjunctival ECM and related conjunctival disorders. Requirements of potential substrates for conjunctival tissue engineering and transplantation are discussed. Biological and synthetic substrates and their components are described in an accompanying review.