Activation of transcription by HIV-1 Tat protein tethered to nascent RNA through another protein
Activation of transcription by HIV-1 Tat protein tethered to nascent RNA through another protein
复制标题
HIV-1 Tat 蛋白通过另一种蛋白与新生 RNA 结合，激活转录
DOI：
10.1038/345640a0
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发表时间：
1990
期刊：
影响因子：
64.8
通讯作者：
Michael R. Green
中科院分区：
文献类型：
--
作者：
C. Southgate;M. Zapp;Michael R. Green
THE human immunodeficiency virus type I (HIV-1) nuclear protein Tat is a potent activator of viral gene transcription1,2. Activation by Tat requires a cis-acting element, the transactivation response (TAR) site, located immediately downstream of the transcription start site35. Several observations suggest that TAR functions as the nascent RNA product of the HIV long-terminal-repeat promoter (for a review, see ref. 6). Indeed, Tat protein and several cellular proteins bind directly to nascent TAR RNA in vitro 710. The significance of these in vitro interactions remains to be established. Here we report that Tat can activate transcription when bound to nascent RNA through the RNA-binding domain of another HIV-1 protein, Rev. Rev is a sequence-specific RNA-binding protein, which interacts with the viral RNA element RRE (refs 11-15). A Tat-Rev fusion protein efficiently activates transcription from an HIV-1 promoter derivative, in which TAR has been replaced by the RRE. We conclude that activation of transcription by Tat can occur by direct binding to nascent RNA, and that the sole function of TAR may be to provide a Tat-binding site. Our results further suggest that cellular proteins that bind specifically to TAR RNA or TAR DNA may not be essential for Tat-responsiveness.
HeLa 细胞核蛋白与人类免疫缺陷病毒反式激活区 RNA 序列的特异性结合。
DOI：
10.1073/pnas.86.13.4858
发表时间：
1989
影响因子：
11.1
作者：
Gaynor,R;Soultanakis,E;Kuwabara,M;Garcia,J;Sigman,DS
通讯作者：
Sigman,DS
影响因子：
10.5
作者：
SELBY, MJ;BAIN, ES;PETERLIN, BM
通讯作者：
PETERLIN, BM
影响因子：
14.9
作者：
KUPPUSWAMY, M;SUBRAMANIAN, T;CHINNADURAI, G
通讯作者：
CHINNADURAI, G