Repair by recombination of DNA containing a palindromic sequence
Repair by recombination of DNA containing a palindromic sequence
复制标题
DOI：
10.1046/j.1365-2958.1997.6071957.x
复制
发表时间：
1997-11-01
影响因子：
3.6
通讯作者：
Pinder, DJ
中科院分区：
文献类型：
--
作者：
Leach, DRF;Okely, EA;Pinder, DJ
我们在这里报道，同源重组功能是维持240 bp染色体反向重复（回文）序列的大肠杆菌细胞生存所必需的。野生型细胞可以成功地复制这个回文，但携带回文的recA、recB或recC突变体是不可存活的。在sbcC突变体中克服了对同源重组的依赖。含有回文的DNA的直接重复拷贝在野生型细胞中迅速分解为单个拷贝，但在sbcC突变体中则不然。我们的研究结果表明，SbcCD核酸酶在回文DNA序列上引入的双链断裂可以通过同源重组修复。修复是保守的，回文保留在修复的染色体中。我们得出的结论是，SbcCD可以攻击二级结构，但修复保留了具有折叠潜力的DNA序列。
We report here that homologous recombination functions ape required for the viability of Escherichia coli cells maintaining a 240 bp chromosomal inverted repeat (palindromic) sequence. Wild-type cells can successfully replicate this palindrome but recA, recB or recC mutants carrying the palindrome are unviable. The dependence on homologous recombination for cell viability is overcome in sbcC mutants. Directly repeated copies of the DNA containing the palindrome are rapidly resolved to single copies in wild-type cells but not in sbcC mutants. Our results suggest that double-strand breaks introduced at the palindromic DNA sequence by the SbcCD nuclease are repaired by homologous recombination. The repair is conservative and the palindrome is retained in the repaired chromosome. We conclude that SbcCD can attack secondary structures but that repair conserves the DNA sequence with the potential to fold.