Retinoic acid and IFN inhibition of cell proliferation is associated with apoptosis in squamous carcinoma cell lines: role of IRF-1 and TGase II-dependent pathways.
Retinoic acid and IFN inhibition of cell proliferation is associated with apoptosis in squamous carcinoma cell lines: role of IRF-1 and TGase II-dependent pathways.
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视黄酸和 IFN 对细胞增殖的抑制与鳞状癌细胞系的细胞凋亡相关：IRF-1 和 TGase II 依赖性途径的作用。
DOI：
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
G. Romeo
中科院分区：
文献类型：
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作者：
Valeria Giandomenico;F. Lancillotti;G. Fiorucci;Z. Percario;R. Rivabene;W. Malorni;E. Affabris;G. Romeo
已知类维生素A和IFN都抑制许多正常和转化细胞的增殖，并对多种癌症（包括鳞状细胞癌）具有体内抗肿瘤作用。由于IFN和全反式维甲酸（RA）的组合可以提高其抗肿瘤效果（取决于组织学起源和细胞的分化状态），我们比较了RA和/或IFN-α 2b的活性与ME 180和SiHa细胞系的生长抑制机制，来自宫颈鳞癌在不同的分化阶段。我们以前报道过，在ME 180细胞系中，联合治疗显著增加了单一药物的生长抑制作用。在这里，我们表明SiHa细胞系似乎比ME 180细胞系对IFN-α 2b更敏感，并且对RA具有抗性，这并不显著抑制SiHa细胞生长。在两种细胞系中明显发生凋亡性细胞死亡的诱导，并与细胞增殖的抑制相关。有趣的是，转录因子IFN调节因子1的诱导与随后诱导的细胞凋亡相关，而TGase I和II的表达则不相关。特别地，TGase I和II在ME 180和SiHa细胞系中表现出差异表达;即，TGase I在ME 180中表达并被RA特异性抑制，而TGase II在SiHa中表达。有趣的是，IFN-α和RA都能够增加该细胞系中TGase II的表达和活性。
Both retinoids and IFNs are known to inhibit proliferation of many normal and transformed cells and to have an in vivo antitumor effect against a variety of cancers, including squamous cell carcinoma. Because the combination of IFNs and all-trans retinoic acid (RA) could improve their antitumor effectiveness (depending on the histological origin and state of differentiation of the cells), we compared the activity of RA and/or IFN-alpha 2b with regard to the mechanism of growth inhibition of ME180 and SiHa cell lines, derived from squamous cervix carcinoma at different stages of differentiation. We reported previously that, in the ME180 cell line, the combined treatment significantly increased the growth inhibitory effect of the single agents. Here, we show that the SiHa cell line appears more sensitive to IFN-alpha 2b than the ME180 cell line, and resistant to RA, which does not significantly inhibit SiHa cell growth. Induction of apoptotic cell death clearly occurs and correlates with the inhibition of cell proliferation in both cell lines. It is interesting that the induction of the transcription factor IFN regulatory factor 1 correlates with the subsequent induction of apoptosis, whereas TGase I and II expression does not. In particular, TGase I and II appear differentially expressed in the ME180 and SiHa cell lines; i.e., TGase I is expressed in ME180 and specifically inhibited by RA, whereas TGase II is expressed in SiHa. It is interesting that both IFN-alpha and RA are able to increase TGase II expression and activity in this cell line.
视黄酸和类视黄醇 X 受体选择性视黄醇与 α-干扰素的组合增强了抑制宫颈癌细胞增殖的功效。
DOI：
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发表时间：
1995
期刊：
Cancer research.
影响因子：
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作者：
Lotan,R;Dawson,MI;Zou,CC;Jong,L;Lotan,D;Zou,CP
通讯作者：
Zou,CP