Intestinal adaptation following spring insertion into a roux limb in mice.
Intestinal adaptation following spring insertion into a roux limb in mice.
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弹簧插入roux肢体后小鼠肠道的适应性变化。
DOI：
10.1016/j.jpedsurg.2020.06.033
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发表时间：
2021-03
影响因子：
2.4
通讯作者：
Dunn JCY
中科院分区：
文献类型：
--
作者：
Portelli KI;Park JB;Taylor JS;Thomas AL;Stelzner M;Martin MG;Dunn JCY
腔内弹簧最近被证明在一个被称为牵张肠发生的过程中延长肠段。我们假设生物相容性弹簧可用于延长去功能化的小鼠小肠，并在分子水平上导致可识别的肠道适应。年龄和体重匹配的C57 BL/6小鼠接受将镍钛合金弹簧加载胶囊手术插入空肠的Roux分支。在初始弹簧放置时以及14天和21天后安乐死时测量节段长度。在划痕时评价Roux肢体的组织学和基因表达，并与未处理的对照节段进行比较。装有压缩弹簧的肠段平均延长了240%，显著长于装有空胶囊或未压缩弹簧的对照段。与没有弹簧的对照组相比，弹簧治疗组小鼠的肌层增厚程度更大。与对照组相比，接受压缩弹簧治疗的小鼠的隐窝深度和Lgr 5+表达更大。在小鼠模型中，将压缩的镍钛合金弹簧插入Roux肢体导致显著的肠延长、平滑肌增厚和Lgr 5+表达。在去功能化小鼠模型中增加小肠长度的能力可用于理解牵张肠形成的机制。
Intraluminal springs have recently been shown to lengthen segments of intestine in a process known as distraction enterogenesis. We hypothesized that biocompatible springs could be used to lengthen defunctionalized murine small intestine and would lead to identifiable intestinal adaptations at the molecular level. Age and weight matched C57BL/6 mice underwent surgical insertion of nitinol spring-loaded capsules into a Roux limb of jejunum. Segment lengths were measured at initial spring placement and at euthanasia after 14 and 21 days. Histology and gene expression of the Roux limb were evaluated at scarification and compared to untreated control segments. Intestinal segments loaded with compressed springs lengthened an average of 240%, which was significantly longer than control segments loaded with either empty capsules or uncompressed springs. Muscularis thickening was greater in spring-treated mice compared to controls without springs. Crypt depth and Lgr5+ expression was greater in mice that received compressed spring treatments when compared to control groups. Insertion of a compressed nitinol spring into a Roux limb results in significant intestinal lengthening, smooth muscle thickening, and Lgr5+ expression in a mouse model. The ability to increase small bowel length in a defunctionalized murine model may be used to understand the mechanism of distraction enterogenesis.
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