INVIVO REGENERATION OF CUT NERVES ENCASED IN SILICONE TUBES - GROWTH ACROSS A 6-MILLIMETER GAP
INVIVO REGENERATION OF CUT NERVES ENCASED IN SILICONE TUBES - GROWTH ACROSS A 6-MILLIMETER GAP
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DOI：
10.1097/00005072-198207000-00004
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发表时间：
1982-01-01
影响因子：
3.2
通讯作者：
VARON, S
中科院分区：
文献类型：
--
作者：
LUNDBORG, G;GELBERMAN, RH;VARON, S
描述了一种用于研究周围神经再生的实验性体内系统，其中横断神经的近端残端通过透明硅胶室朝向远端残端再生。物理分离允许检查远端残端对再生纤维的体液和/或细胞影响的影响，然后再侵入远端节段本身。切除大鼠坐骨神经的一小段，留下6 mm的间隙，然后用圆柱形硅胶室包裹。第1周内，神经干沿着腔室中轴再生，桥接近端和远端残端之间的差距。当远端神经残端从室的远端开口省略时，仅具有少量小口径纤维的薄结构延伸穿过差距。在每种情况下，再生神经都表现为完全被透明液体包围的索状结构，这一特征允许容易地收集细胞外液以分析其化学性质和生物活性。该特征还允许在体内操纵发生神经再生的体液环境。
An experimental in vivo system was described for studying peripheral nerve regeneration, in which the proximal stump of a transected nerve regrows through a transparent silicone chamber toward the distal stump. Physical separation permits examination of the effects of the humoral and/or cellular influences from the distal stump on regenerating fibers before they invade the distal segment itself. A small segment of the rat sciatic nerve was resected, leaving a 6 mm gap which was then encased by a cylindrical silicone chamber. Within the 1st weeks, a nerve trunk regenerated along the central axis of the chamber bridged the gap between the proximal and distal stumps. When the distal nerve stump was omitted from the distal opening of the chamber, only a thin structure with a few small-caliber fibers extended across the gap. In each instance regenerating nerve appeared as a cord-like structure completely surrounded by clear fluid, a feature which permits easy collection of the extracellular fluid for analysis of its chemical properties and biological activity. This feature also allows in vivo manipulation of the humoral environment in which nerve regeneration occurs.