Heat shock factor regulates VDUP1 gene expression
Heat shock factor regulates VDUP1 gene expression
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DOI：
10.1016/j.bbrc.2004.01.047
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发表时间：
2004-03-05
影响因子：
3.1
通讯作者：
Choi, I
中科院分区：
文献类型：
--
作者：
Kim, KY;Shin, SM;Choi, I
维生素D3上调蛋白1(VDUP1)是一种与硫氧还蛋白(Trx)相互作用的蛋白，是Trx的天然拮抗剂。它的表达受各种胁迫的调节，包括ROS、UV和热休克。在本研究中，我们观察到在高密度和去血清培养条件下，VDUP1在BOSC细胞中可被诱导表达。为了确定与胁迫诱导VDUP1相关的转录因子，克隆了VDUP1的启动子区域。通过对启动子区域不同缺失的质粒进行报告分析和顺式元件的分析，鉴定了热休克因子元件(HSE)。HSE的缺失取消了VDUP1启动子在应激条件下的转录活性，并通过凝胶位移和超位移实验证实了热休克因子(HSF)与HSE的结合。此外，HSF的增强表达或热休克增加了内源性VDUP1的转录，这表明HSF是一个重要的转录因子，参与了高密度和血清剥夺等应激条件下VDUP1表达的上调。(C)2004 Elsevier Inc.保留所有权利。
The vitamin D3 up-regulated protein 1 (VDUP1) is identified as interacting protein with thioredoxin (TRX) and functions as a natural antagonist of TRX. Its expression is regulated by various stresses including ROS, UV, and heat shock. In the present study, we observed an inducible expression of VDUP1 in Bosc cells by high density and serum deprivation cultures. To determine transcription factors associated with the induction of VDUP1 by stresses, the promoter region of VDUP1 was cloned. Through reporter assays with plasmids having various deletion of its promoter region and analysis of putative cis-elements, heat shock factor element (HSE) was identified. The deletion of HSE abolished transcriptional activity of VDUP1 promoter by stresses and the binding of heat shock factor (HSF) to HSE was confirmed by gel-shift and supershift assays using nuclear extracts prepared from stressed Bosc cells. Also, the enforced expression of HSF or heat shock increased the transcription of endogenous VDUP1 These imply that HSF is an important transcription factor involved in up-regulation of VDUP1 expression by stresses such as high density and serum deprivation cultures. (C) 2004 Elsevier Inc. All rights reserved.